We narrowed to 8,146 results for: Lif

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pcDN3.1(+)-CaNARi

Plasmid

#64848

Purpose

FRET-based calcineurin (CaN) activation biosensor.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Tags

Cerulean and Venus

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCZGY3163

Plasmid

#101248

Purpose

GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

gfp11::rps-18 (rps-18 Nematode, Synthetic)

Use

Gateway

Tags

GFP11

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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BFP-Rab5CA (Q79L)

Plasmid

#205705

Purpose

Mammalian expression of BFP-tagged mutant Rab5CA

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

RAB5A (RAB5A Human)

Expression

Mammalian

Mutation

Q79L

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pACS131

Plasmid

#204596

Purpose

Histone H3.1K27M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 K27M (H3C1 Human)

Use

Lentiviral

Tags

human histone H3.1-V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-NLS-TurboID

Plasmid

#163849

Purpose

In vitro transcription of nls-TurboID. Parton lab clone JST

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

In vitro transcription of rna

Tags

nls

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDK521

Plasmid

#134204

Purpose

Baculovirus expresion of Astrin, LC8, and SKAP

Depositor

Iain Cheeseman

Article

Kern et al Elife. 2017 Aug 25;6. pii: 26866. doi: 1

Insert

Astrin 1-693-sGFP-His BroAstrin and LC8 and AltSKAP in pACE1

Expression

Insect

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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N-GFP-Dcp2

Plasmid

#163585

Purpose

express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

DCP2 promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-UbiC-GFP-ps

Plasmid

#183712

Purpose

GFP with palmitoylation signal

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

GFP with palmitoylation signal

Use

Lentiviral

Tags

palmitoylation signal

Promoter

UbiC

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pN3-mTagBFP-pHluorin-CAAX

Plasmid

#178345

Purpose

Express a pH insensitive blue fluorencent protein mTagBFP fused to the N-terminal of a membrane targeted pH sensitive green fluorescent protein pHluorin-CAAX under CMV promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

mTagBFP-pHluorin-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFsynW TeTx-LC

Plasmid

#197284

Purpose

Encodes for viral expression of the light chain of tetanus toxin.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

Tetanus toxin light chain

Use

Lentiviral

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-GFP

Plasmid

#175757

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

GFP

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-tdMCP-msfGFP(V206K)-GBI

Plasmid

#205163

Purpose

express tdMCP-msfGFP(V206K)-GBI

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

tdMCP-msfGFP(V206K)-GBI

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pQIq_MRGS_HIS8_(27F3)_FLAG

Plasmid

#199615

Purpose

Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27F3

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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epB_CAG_TetRFlag-nls-tdTom_DEx4

Plasmid

#119909

Purpose

ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

TetRFlag-nls-tdTom

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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UAS:TVA-mCherry

Plasmid

#187823

Purpose

Expression of a fusion of the transmembrane protein TVA and the red fluorescent protein mCherry under UAS control.

Depositor

Rainer Friedrich

Article

Satou et al Elife. 2022 Jul 22;11. pii: 77153. doi:

Insert

TVA-mCherry

Expression

Bacterial

Promoter

5xUAS

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMiniT:GFP-Gephyrin TKIT donor

Plasmid

#169433

Purpose

Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-jGCaMP7b-XN

Plasmid

#178362

Purpose

Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.

Depositor

Xiaodong Liu

Article

Geng et al Elife. 2022 Oct 5;11:e76691. doi: 10.755

Insert

jGCaMP7b-XN

Expression

Mammalian

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-RILP

Plasmid

#74600

Purpose

used for expression in E. coli and purification

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Rab-interacting lysosomal protein (RILP Human)

Tags

FLAG, StrepII, and superfolder GFP

Expression

Bacterial

Promoter

T7

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-FLAG-R-Ras2

Plasmid

#102335

Purpose

Human R-Ras2 with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Elife. 2017 Apr 13;6. pii: e25158. doi:

Insert

RRAS2 (RRAS2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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