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Plasmid#205978PurposeFLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas11/Cse2 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTR AE Clover
Plasmid#90240PurposeLentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (AE mutant)
UseLentiviralTagsmCloverExpressionMammalianMutationPromoterPGKAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
(194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)
Plasmid#160742PurposeExpresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.DepositorInsertHA-nEPEA-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_GABPA
Plasmid#64253PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPADepositorInsertGABPA Homology Arms (GABPA Human)
UseTags3X Flag P2A NeoExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_KMT2B
Plasmid#101070PurposeDonor vector for 3' FLAG tag of human KMT2BDepositorInsertKMT2B homology arms (KMT2B Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-pEGFP-C1
Plasmid#166737PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, BikDepositorInsertBik (BIK Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_KMT2B_2
Plasmid#101075PurposeEncodes gRNA for 3' target of human KMT2BDepositorInsertgRNA against KMT2B (KMT2B Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_KMT2B_1
Plasmid#101074PurposeEncodes gRNA for 3' target of human KMT2BDepositorInsertgRNA against KMT2B (KMT2B Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (72P)
Plasmid#62083PurposeExpression of human p53 (1-73) (72P) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHIsExpressionBacterialMutationcontains TAD residues 1-73; 72PPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFETCH_ATF1
Plasmid#64689PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human ATF1DepositorInsertATF1 Homology Arms (ATF1 Human)
UseTags3X Flag P2A NeoExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-MCL1S159A
Plasmid#21606DepositorInsertMCL-1 S159A (MCL1 Human)
UseTagsExpressionMammalianMutationSerine 159 mutated to Alanine, abolishing phosph…PromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_FOXM1_iso1
Plasmid#135734PurposeDonor vector for 3' FLAG tag of human FOXM1_iso1DepositorInsertFOXM1_iso1 Homology arms (FOXM1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458_FOXM1_iso1_2
Plasmid#135753PurposeEncodes gRNA for 3' target of human FOXM1_iso1DepositorInsertFOXM1_iso1 gRNA (FOXM1 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458_FOXM1_iso1_1
Plasmid#135752PurposeEncodes gRNA for 3' target of human FOXM1_iso1DepositorInsertFOXM1_iso1 gRNA (FOXM1 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (72R)
Plasmid#62082PurposeExpression of human p53 (1-73) (72R) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHIsExpressionBacterialMutationcontains TAD residues 1-73; 72RPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMA3431
Plasmid#46876PurposeA lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3 mutant R592QDepositorInsertsoluble guanylate cyclase 1alpha 3 (Gucy1a3 Rat)
UseLentiviralTagsMycExpressionMutationR592QPromoterTRE-TightAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ARID4B
Plasmid#101072PurposeDonor vector for 3' FLAG tag of human ARID4BDepositorInsertARID4B homology arms (ARID4B Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_GABPB1_iso2_1
Plasmid#104041PurposeEncodes gRNA for 3' target of human GABPB1_iso2 along with Cas9 with 2A GFPDepositorInsertGABPB1_iso2 gRNA (GABPB1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only