Addgene

We narrowed to 2,116 results for: Pam

Showing: 981 - 1000 of 2116 results

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pMXS-IRES-BLAST flag-CAD T1348A

Plasmid

#188120

Purpose

Expresses C-terminal flag-tagged human CAD T1348A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCITETtTERT

Plasmid

#27660

Depositor

Kathleen Collins

Article

Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):25

Insert

Tetrahymena TERT

Use

Reticulocyte lysate

Tags

Expression

Mutation

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pET15p65

Plasmid

#27661

Depositor

Kathleen Collins

Article

Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):25

Insert

Tetrahymena telomerase holoenzyme p65

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pCITEp65

Plasmid

#27659

Depositor

Kathleen Collins

Article

Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):25

Insert

Tetrahymena telomerase holoenzyme p65

Use

Reticulocyte lysate

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pIA030

Plasmid

#200424

Purpose

thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al bioRxiv 2023

Insert

LacI

Use

Tags

PAmCherry

Expression

Bacterial

Mutation

Promoter

Ppen

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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RFP-HA-DAT

Plasmid

#90265

Purpose

Expresses RFP-HA-DAT in mammalian cells

Depositor

Alexander Sorkin

Article

Caltagarone et al Mol Cell Neurosci. 2015 Sep;68:120-30. d

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT)

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-DAT

Plasmid

#90228

Purpose

Expresses human DAT in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Biol Chem. 2003 Jul 25;278(30):28274-8

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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pXD70EN25-Pct5-(–)-Eddh(Gp3C)

Plasmid

#233877

Purpose

Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-loxP-GRAB_DA2m-loxP

Plasmid

#189751

Purpose

Excludes sensor expression in Cre-positive neurons

Depositor

Yulong Li

Article

Dai et al Cell Rep. 2022 Aug 23;40(8):111246. doi:

Insert

Dopamine sensor GRAB_DA2m

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT

Plasmid

#90244

Purpose

Expresses YFP-HA-DAT in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2006 Aug 2;26(31):8195-205.

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

residues 193–203 of human DAT were replaced in YF…

Promoter

CMV

Available since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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pcihygroflagsynhDAT

Plasmid

#19990

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

Dopamine transporter (SLC6A3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1wt-PA-mCherry

Plasmid

#138252

Purpose

Wild-type CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry).

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1-PAmCherry

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-DAT R60A

Plasmid

#90247

Purpose

Expresses YFP-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Ma et al Sci Rep. 2017 Jul 14;7(1):5399. doi: 10.

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

mutated R60 to A in human DAT cDNA

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT W63A

Plasmid

#90246

Purpose

Expresses YFP-HA-DAT W63A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated W63 to A

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT R60A

Plasmid

#90245

Purpose

Expresses YFP-HA-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated R60 to A

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)

Plasmid

#225307

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GST_LIM1224

Plasmid

#182041

Purpose

For expression of human paxillin LIM domains 1, 2, 2 and 4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM1224 (PXN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

LIM domains 1, 2, 2 and 4

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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GST_LIM1334

Plasmid

#182040

Purpose

For expression of human paxillin LIM domains 1, 3, 3 and 4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM1334 (PXN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

LIM domains 1, 3, 3 and 4

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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