Addgene

We narrowed to 2,701 results for: SIR

Showing: 981 - 1000 of 2701 results

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer10b-HA-KRAS WT CO SR

Plasmid

#164926

Purpose

Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequence

Depositor

Ji Luo

Article

Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

Insert

KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)

Use

Lentiviral

Tags

HA tag

Expression

Mutation

9 Valine codons are optimized and siRNA targetin…

Promoter

Ubc promoter

Available since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10b-HA-KRAS G12V CO SR

Plasmid

#164928

Purpose

Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence

Depositor

Ji Luo

Article

Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

Insert

KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)

Use

Lentiviral

Tags

HA tag

Expression

Mutation

Glycine 12 changed to valine; 9 Valine codons are…

Promoter

Ubc promoter

Available since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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p184.3

Plasmid

#44521

Depositor

Michael Grunstein

Article

Hecht et al Cell. 1995 Feb 24;80(4):583-92.

Insert

Histone 3 N-terminus

Use

Tags

GST

Expression

Bacterial

Mutation

aa1-46

Promoter

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
p297.1

Plasmid

#44520

Depositor

Michael Grunstein

Article

Hecht et al Cell. 1995 Feb 24;80(4):583-92.

Insert

Histone 2B2 aa 1-35

Use

Tags

GST

Expression

Bacterial

Mutation

aa 1-35

Promoter

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
p296.2

Plasmid

#44519

Depositor

Michael Grunstein

Article

Hecht et al Cell. 1995 Feb 24;80(4):583-92.

Insert

Histone 2A1 aa 1-35

Use

Tags

GST

Expression

Bacterial

Mutation

aa 1-35

Promoter

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
p109.1

Plasmid

#44518

Depositor

Michael Grunstein

Article

Hecht et al Cell. 1995 Feb 24;80(4):583-92.

Insert

Histone 4

Use

Tags

GST

Expression

Bacterial

Mutation

contains full length (aa 1-102)

Promoter

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
p107.1

Plasmid

#44517

Depositor

Michael Grunstein

Article

Hecht et al Cell. 1995 Feb 24;80(4):583-92.

Insert

Histone 4 N-terminus aa1-34

Use

Tags

GST

Expression

Bacterial

Mutation

aa1-34 of H4

Promoter

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
pRL-TK CXCR4 4x

Plasmid

#11307

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

4 bulged bind sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pRL-TK CXCR4 6x

Plasmid

#11308

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

6 bulged bind sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pRL-TK CXCR4 1P

Plasmid

#11309

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

1 perfect bind site for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pPS2971

Plasmid

#8926

Depositor

Pamela Silver

Article

Devroe et al BMC Biotechnol. 2002 Aug 28. 2():15.

Insert

NDR1 (STK38 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pGL3 CXCR4 1P

Plasmid

#11310

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

1 perfect binding site for CXCR4 siRNA antisense

Use

Luciferase

Tags

Pp-luc

Expression

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pGL3 CXCR4 2P

Plasmid

#11311

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

2 perfect binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Pp-luc

Expression

Mutation

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRL-TK CXCR4 2x

Plasmid

#11306

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

2 bulged bind sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3 CXCR4 3P

Plasmid

#11312

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

3 perfect binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Pp-luc

Expression

Mutation

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pPS2972

Plasmid

#8942

Depositor

Pamela Silver

Article

Devroe et al BMC Biotechnol. 2002 Aug 28. 2():15.

Insert

NDR1 (STK38 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pICH47732:FCP:NAT

Plasmid

#85984

Purpose

Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

NAT

Use

Golden gate assembly

Tags

Expression

Mutation

Promoter

FCP

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFX21

Plasmid

#44533

Depositor

Michael Grunstein

Article

Xu et al Mol Cell. 2007 Sep 21;27(6):890-900.

Insert

SIR2-6xHis

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only