Addgene

We narrowed to 11,261 results for: Vars

Showing: 981 - 1000 of 11261 results

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MAC3-N-GFP-NES

Plasmid

#222606

Purpose

Expression of MAC3-tagged GFP-NES (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' nuclear exclusion sequence (NES)

Use

Tags

MAC3

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-NTRK3

Plasmid

#222612

Purpose

Expression of MAC3-tagged NTRK3

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

NTRK3 (NTRK3 Human)

Use

Tags

MAC3

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-N-GFP-NLS

Plasmid

#222607

Purpose

Expression of MAC3-tagged GFP-NLS (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' nuclear localizaition sequence (NLS)

Use

Tags

MAC3

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-C-GFP-NES

Plasmid

#222603

Purpose

Expression of MAC3-tagged GFP-NES (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' nuclear exclusion sequence (NES)

Use

Tags

MAC3

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-N-GFP-Myr

Plasmid

#222608

Purpose

Expression of MAC3-tagged GFP-Myr (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' myristoylation sequence (Myr)

Use

Tags

MAC3

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-C-GFP-Myr

Plasmid

#222605

Purpose

Expression of MAC3-tagged GFP-Myr (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' myristoylation sequence (Myr)

Use

Tags

MAC3

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Strep-TGT-His

Plasmid

#206495

Purpose

Expresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His Tag

Depositor

Neal Devaraj

Article

Tota et al J Am Chem Soc. 2023 Apr 12;145(14):8099-

Insert

E. coli tRNA Guanine Transglycosylase

Use

Tags

His and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI

Plasmid

#192113

Purpose

Expresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Insert

SARS-CoV-2-NTD-BA.4/5 (S )

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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DUX4_KBM pDonor221

Plasmid

#187802

Purpose

DUX4 with KBM (p. E414-L424 deletion) entry clone

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

DUX4-KBM (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

p. E414-L424 deletion

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_DUX4_KBM

Plasmid

#187800

Purpose

DUX4 with KBM (p. E414-L424 deletion) with MAC-C tag

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

DUX4-KBM (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

p. E414-L424 deletion

Promoter

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_NTRK3

Plasmid

#187796

Purpose

MAC-tagged gene expression of human NTRK3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

NTRK3 (NTRK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_PAX2

Plasmid

#187736

Purpose

MAC-tagged gene expression of human PAX2

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

PAX2 (PAX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_RET

Plasmid

#187775

Purpose

MAC-tagged gene expression of human RET

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

RET (RET Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_TLX3

Plasmid

#167807

Purpose

Expresses TLX3 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

TLX3 (TLX3 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_PAX9

Plasmid

#167789

Purpose

Expresses PAX9 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

PAX9 (PAX9 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_TLX2

Plasmid

#167806

Purpose

Expresses TLX2 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

TLX2 (TLX2 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCSJ182

Plasmid

#159723

Purpose

Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

GID4 (VID24 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCSJ392

Plasmid

#159722

Purpose

Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

GID10 (GID10 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1

Plasmid

#145369

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Use

Tags

His and eGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_4

Plasmid

#102966

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_4 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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