We narrowed to 2,134 results for: dcas9

1. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

   Plasmid

   #84261

   Purpose

   Expresses AND gate ABA/GA-inducible VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9
   PYL1-VPR-p2a-GID1-ABI
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84239

   Purpose

   Expresses ABA-inducible VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sp dCas9
   PYL1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

   Plasmid

   #84243

   Purpose

   Expresses ABA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sa dCas9
   PYL1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84241

   Purpose

   Expresses ABA-inducible KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sp dCas9
   PYL1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

   Plasmid

   #84257

   Purpose

   Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9-ABI
   GID1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

   Plasmid

   #187959

   Purpose

   Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Inserts

   Blasticidin resistance
   FKBP12_F36V
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

   Plasmid

   #106895

   Purpose

   Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9-ABI
   GID1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

   Plasmid

   #187344

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   KRAB WSR7EEE

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

   Plasmid

   #187327

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   KRAB WSR7EEE

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJT341 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

    Plasmid

    #195540

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

    Plasmid

    #168290

    Purpose

    CRISPR-activator Suntag-P65-HSF1 (SPH)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    dCas9, Suntag-P65-HSF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

    Plasmid

    #107307

    Purpose

    Encoding an SPH activation system

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

    Plasmid Kit

    #1000000062

    Purpose

    Accessory pack for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either dCas9 or dCas9-FokI fusion; Kit #1000000055 is required to use this kit

    Depositor

    Takashi Yamamoto

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCK668

    Plasmid

    #192637

    Purpose

    Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    SoxS has R93A and S101A mutations and dCas9-NG ha…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. iB22 PB-zim3-mycNLS-mApple

    Plasmid

    #204718

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. iC10 PB-zim3-mycNLS-mApple-hygro

    Plasmid

    #204719

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRR2

    Plasmid

    #166097

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pITI1

    Plasmid

    #166094

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIF4

    Plasmid

    #196069

    Purpose

    Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae

    Depositor

    Kabin Xie

    Article

    Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MoRP27 promoter

    Available since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIF3

    Plasmid

    #196068

    Purpose

    Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU3 promtoer in M. oryzae

    Depositor

    Kabin Xie

    Article

    Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MoRP27 promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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