We narrowed to 22,151 results for: tes

1. pcDNA3.1(+)-ExRai-AKAR2(T/A)

   Plasmid

   #161754

   Purpose

   Negative-control mutant for ExRai-AKAR2 biosensor.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   ExRai-AKAR2(T/A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHDM-VSV-G

   Plasmid

   #164440

   Purpose

   Encodes VSV-G protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   VSV-G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

   Plasmid

   #61590

   Purpose

   Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

   Depositor

   David Komander

   Article

   Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

   Insert

   TRABID (ZRANB1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-244 and aa 698-708.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOPINK-OTUD2 (full-length, aa 1-348)

   Plasmid

   #61409

   Purpose

   Expresses human OTUD2 (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD2 (YOD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHDM-Tat1b

   Plasmid

   #164442

   Purpose

   Encodes HIV-1 Tat accessory protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   HIV-1 Tat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOPINK-OTUD3 (OTU, aa 52-209)

   Plasmid

   #61412

   Purpose

   Expresses human OTUD3 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD3 (OTUD3 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-51 and aa 210-398.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

   Plasmid

   #132771

   Purpose

   Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   Cyclic GMP-AMP synthase (CGAS Human)
   

   Use

   Lentiviral

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   E225A D227A

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. single polypeptide FPX biosensor for calcium ion

   Plasmid

   #60887

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for calcium ion
   

   Use

   Tags

   HindIII site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTet-O-ATOH1-T2A-PuroR

   Plasmid

   #162342

   Purpose

   Lentiviral expression of ATOH1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   ATOH1 (ATOH1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag‐mTim3

    Plasmid

    #49208

    Purpose

    expresses Flag tagged mouse Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (Havcr2 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    no Tim3 signal sequence

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP Abi1

    Plasmid

    #74905

    Purpose

    Expresses human Abi1 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

    Insert

    Abi1 (ABL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p5E-gfap

    Plasmid

    #75024

    Purpose

    Can be used to drive expression in zebrafish astrocytes.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    glial fibrillary acidic protein promoter (gfap Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    gfap

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EGFP-HsNRF2 (NFE2L2)

    Plasmid

    #194303

    Purpose

    Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-pmExRai-AKAR2

    Plasmid

    #161755

    Purpose

    Plasma membrane-targeted ExRai-AKAR2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    Lyn-ExRai-AKAR2
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTet-O-ASCL1-T2A-PuroR

    Plasmid

    #162345

    Purpose

    Lentiviral expression of ASCL1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    ASCL1 (ASCL1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-Miro1 S156A

    Plasmid

    #127588

    Purpose

    Phospho-mutant of S156

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S156A

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2plus-mKate2

    Plasmid

    #162624

    Purpose

    Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BNLS

    Plasmid

    #50836

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP B
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-mitoExRai-AKAR2

    Plasmid

    #161757

    Purpose

    ExRai-AKAR2 targeted to the outer mitochondrial membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    DAKAP-ExRai-AKAR2
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p5E--3mnx1

    Plasmid

    #74632

    Purpose

    Can be used to drive expression specifically in zebrafish motor neurons

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    -3mnx1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge