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Showing: 10021 - 10040 of 18429 results

  1. L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10

    Plasmid
    #219701
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1
    Depositor
    Jennifer Nemhauser
    Insert
    pAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219701-plasmid-map-sequence-id-435731

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  2. pIND4-RC-M210TAG-A7

    Plasmid
    #223015
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for 3-nitrotyrosine
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    A7 RS
    pylT tRNA
    Tags
    6xHis
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available Since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    223015-plasmid-map-sequence-id-444740

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  3. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

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  4. gWiz_BM40-vMAT2-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218442
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vMAT2 Fab domain
    Depositor
    Axel Brunger
    Insert
    vMAT2-Vh-hIgG1-CH4
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218442-plasmid-map-sequence-id-431084

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  6. ER-FAST-IRES-iRFP670

    Plasmid
    #216344
    Purpose
    For testing localization of pFAST and iRFP670 proteins to endoplasmic reticulum
    Depositor
    Alison Tebo
    Insert
    PRL_pFAST-IRES-CANX_iRFP670
    Tags
    myc for FAST and iRF670 proteins
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available Since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216344-plasmid-map-sequence-id-427396

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  8. GBH-R-gcryBFP.1

    Plasmid
    #187995
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker gamma crystallin BFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    187995-plasmid-map-sequence-id-380758

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  9. GBH-R-gcryGFP.3

    Plasmid
    #188000
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188000-plasmid-map-sequence-id-381121

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  10. PAS_RvSAHS6

    Plasmid
    #215647
    Purpose
    Expresses SUMO-tagged RvSAHS6 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS6
    Tags
    SUMO
    Expression
    Bacterial
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215647-plasmid-map-sequence-id-426094

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  11. PAS_RvSAHS4

    Plasmid
    #215646
    Purpose
    Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS4
    Tags
    SUMO
    Expression
    Bacterial
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215646-plasmid-map-sequence-id-426091

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  12. pcDNA3.1_hMed25

    Plasmid
    #206958
    Purpose
    The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    206958-plasmid-map-sequence-id-422373

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  13. pCHA_OROV_NSs

    Plasmid
    #208939
    Purpose
    Expresses C-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 66 bp from 5' end and deleted 415 b…
    Promoter
    CMV
    Available Since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208939-plasmid-map-sequence-id-419030

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  14. pNHA_SBV_NSs

    Plasmid
    #208932
    Purpose
    Expresses N-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 50 bp from 5' end (including start c…
    Promoter
    CMV
    Available Since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208932-plasmid-map-sequence-id-419004

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  16. pAttB-Tub-HA-Wnk

    Plasmid
    #203968
    Purpose
    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression
    Depositor
    Andreas Jenny
    Insert
    Wnk (most similar to RO variant) (Wnk Fly)
    Tags
    HA
    Expression
    Insect
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203968-plasmid-map-sequence-id-403124

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  17. huntingtin_V2095-V3138_CbioCflag

    Plasmid
    #194764
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV and P10
    Available Since
    July 27, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194764-plasmid-map-sequence-id-402574

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  18. huntingtin_T97-M2069_NbioCflag

    Plasmid
    #194763
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV and P10
    Available Since
    July 26, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194763-plasmid-map-sequence-id-402570

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  19. huntingtin_V2095-V3138_NbioCflag

    Plasmid
    #194765
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV and P10
    Available Since
    July 26, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194765-plasmid-map-sequence-id-402569

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  20. pCI_E01_mini-mAgrin-Myc-His-WPRE__LG1-3

    Plasmid
    #198141
    Purpose
    Express mini-Agrin in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    mini-Agrin (Agrn Mouse)
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available Since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    198141-plasmid-map-sequence-id-390123

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Showing: 10021 - 10040 of 18429 results