We narrowed to 118,701 results for: MPI

1. pCW57.1-GFP_mmu-miR-16-5p_sponge

   Plasmid

   #132624

   Purpose

   Expresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTR

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-16-5p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p159-RV-syn-dio-mScarlet-T2A

   Plasmid

   #185700

   Purpose

   Retroviral vector expression cre-dependent membrane tagged mScarlet-T2A driven by the rat Synapsin promoter (with XhoI and PacI at 3'end of T2A to clone a gene of interest)

   Depositor

   Lynette Lim

   Insert

   mScarlet
   

   Use

   Retroviral

   Tags

   mScarlet palmitoylation with T2A on 3'-end

   Promoter

   Synapsin

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2iD6Hoxa13bER4(#686)

   Plasmid

   #184101

   Purpose

   cyclofen-inducible fish Hoxa13b activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   ZfHoxa13b-ERT2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMHE-TNKS-2-M1054V

   Plasmid

   #138538

   Purpose

   For making TNKS-2-M1054V RNA

   Depositor

   Carmen Williams

   Insert

   Tankyrase 2 (TNKS2 Human)
   

   Use

   In vitro transcription

   Tags

   FLAG

   Mutation

   Changed methionine 1054 to valine

   Promoter

   T7

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2iD6Hoxa13aER4(#685)

   Plasmid

   #184100

   Purpose

   cyclofen-inducible fish Hoxa13a activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   ZfHoxa13a-ERT2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. UTP15:PBC028-C01:C245785

   Plasmid

   #183973

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   UTP15 (UTP15 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. LIPS:SDC101a-A08:C34411

   Plasmid

   #183978

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   LIPS
   

   Use

   Baculovirus expresssion

   Tags

   C-terminal 8xHis tag:EFVEHHHHHHHH and N-termina…

   Promoter

   Polyhedrin

   Available Since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q

   Plasmid

   #147279

   Purpose

   Insect Expression of DmNot3-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmNot3-dsRNAres (Not3 Fly)
   

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE31-6xHis-Countdown

   Plasmid

   #182299

   Purpose

   E. coli plasmid encoding Countdown with a hexahistidine tag

   Depositor

   Andrew York

   Article

   Seidel et al (2021), doi: 10.5281/zenodo.5810930

   Insert

   Countdown
   

   Tags

   histidine tag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE31-6xHis-pHCountdown

    Plasmid

    #182300

    Purpose

    E. coli plasmid encoding pH-Countdown with a hexahistidine tag

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    pH Countdown
    

    Tags

    histidine tag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G

    Plasmid

    #146359

    Purpose

    Insect Expression of DmDCP2-PB

    Depositor

    Elisa Izaurralde

    Insert

    DmDCP2-PB (DCP2 Fly)
    

    Expression

    Insect

    Mutation

    Contains an in-frame insertion that adds a Q afte…

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. loxP-T2A-Myr-Casp8-ERT2-rox

    Plasmid

    #182362

    Purpose

    Shuttle vector for T2A-Myr-Casp8-ERT2

    Depositor

    Mario Capecchi

    Insert

    loxP-T2A-Myr-Casp8-ERT2-rox
    

    Use

    Cre/Lox and Mouse Targeting; Dre / rox

    Expression

    Mammalian

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. loxP-T2A-Myr-DeadCasp8-ERT2-rox

    Plasmid

    #182361

    Purpose

    Shuttle vector for T2A-Myr-DeadCasp8-ERT2

    Depositor

    Mario Capecchi

    Insert

    loxP-T2A-Myr-DeadCasp8-ERT2-rox
    

    Use

    Cre/Lox and Mouse Targeting; Dre / rox

    Expression

    Mammalian

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnEA-NpG-HsNot11_257-498_AF

    Plasmid

    #148651

    Purpose

    Bacterial Expression of HsNot11_257-498

    Depositor

    Elisa Izaurralde

    Insert

    HsNot11_257-498 (CNOT11 Human)
    

    Expression

    Bacterial

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDZ2FE_Rev3_1873-2025

    Plasmid

    #105672

    Purpose

    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein

    Depositor

    Thanos Halazonetis

    Insert

    Rev3L (REV3L Human)
    

    Tags

    2X Flag tag

    Expression

    Mammalian

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT-NTAP-DmNot3_J

    Plasmid

    #146651

    Purpose

    Insect Expression of DmNot3

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3 (Not3 Fly)
    

    Expression

    Insect

    Mutation

    One silent and three non silent mutations E90G, K…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-EOLA1/CXORF40A

    Plasmid

    #180776

    Purpose

    Expresses EOLA1/CXORF40A in mammalian cells

    Depositor

    Douglas Feldman

    Insert

    EOLA1 (EOLA1 Human)
    

    Tags

    SBP-Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pnEA-NpH-HsNot1iso1_4-302_Q

    Plasmid

    #147296

    Purpose

    Bacterial Expression of HsNot1iso1_4-302

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1iso1_4-302 (CNOT1 Human)
    

    Expression

    Bacterial

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-lambdaN-HA-DmBancal-del192-424-V5His6_AB

    Plasmid

    #148297

    Purpose

    Insect Expression of DmBancal-del192-424

    Depositor

    Elisa Izaurralde

    Insert

    DmBancal-del192-424 (HnRNP-K Fly)
    

    Expression

    Insect

    Mutation

    6 silent mutations compared to the sequence of is…

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIneo-RLuc_C

    Plasmid

    #146090

    Purpose

    Mammalian Expression of Rluc

    Depositor

    Elisa Izaurralde

    Article

    Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

    Insert

    Rluc
    

    Expression

    Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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