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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,050 results for: nin

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Showing: 10041 - 10060 of 66050 results

  3. pDB023f1

    Plasmid
    #99218
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99218-plasmid-map-sequence-id-192402

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  5. p007ampGFP

    Plasmid
    #58535
    Purpose
    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.
    Depositor
    Jaroslaw Bryk
    Publication
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Expression
    Bacterial
    Promoter
    T5 between two lac operators
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58535-plasmid-map-sequence-id-90574

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  6. RACGAP1

    Plasmid
    #38807
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RACGAP1 (Racgap1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

    Plasmid
    #203652
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
    Depositor
    Liam Holt
    Publication
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire-IRES-H2B-mCherry
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203652-plasmid-map-sequence-id-402483

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  12. pCAT100

    Plasmid
    #180512
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Publication
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180512-plasmid-map-sequence-id-359730

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  14. DT041A

    Plasmid
    #159402
    Purpose
    Constitutive murine TrkB expression in mammalian host, controlled under cytomegalovirus (CMV) promoter and enhancer
    Depositor
    Sheref Mansy
    Publication
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pLenti-CMV-TrkB (Ntrk2, Mouse) (Ntrk2 Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    159402-plasmid-map-sequence-id-324292

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  17. xCas9

    Plasmid
    #138565
    Purpose
    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    xCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138565-plasmid-map-sequence-id-274797

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  18. pUF1-dCas9-Sir2a

    Plasmid
    #122510
    Purpose
    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Publication
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Insert
    dCas9-Sir2a
    Use
    CRISPR
    Tags
    3*FLAG
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122510-plasmid-map-sequence-id-243592

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  20. zCD2

    Plasmid
    #122031
    Purpose
    Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.
    Depositor
    David Christianson
    Publication
    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi
    Insert
    Zebrafish HDAC6 Catalytic Domain 2
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    122031-plasmid-map-sequence-id-240596

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Showing: 10041 - 10060 of 66050 results