We narrowed to 61,377 results for: promoter

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mNeonGreen-DEVD-NanoLuc

Plasmid

#98287

Purpose

caspase-3 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro-CAG-GCaMP6s

Plasmid

#120896

Purpose

Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

Depositor

Xiaojun Lian

Article

Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

Insert

GCaMP6s

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-EDB in pMAX

Plasmid

#120403

Purpose

enables eukaryotic expression of human EDB (or EIIIB) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Article

Kraft et al J Mol Med (Berl). 2016 May;94(5):567-81.

Insert

EDB-fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-eBE-Y130F

Plasmid

#113423

Purpose

Expresses hA3A-eBE-Y130F in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y130F

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1-3xFlag-7xKQ

Plasmid

#111576

Purpose

Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

lysine to glutamine mutation at the following ami…

Promoter

CMV

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D V5-His-TOPO-KGA.wt

Plasmid

#110332

Purpose

Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

KGA glutaminase (GLS Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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Lifeact-7-iRFP670

Plasmid

#103032

Purpose

Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Tags

iRFP670

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

Plasmid

#104571

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

human codon optimized ‘dead’ Cpf1 fused to four DmrA domains

Use

CRISPR

Tags

NLS-3xHA-3xFLAG-4xDmrA

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRVL-4

Plasmid

#104582

Purpose

Contains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human CL kappa

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-mTurquoise2

Plasmid

#104059

Purpose

AAV expression of Cre-dependent reporter mTurquoise2

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mTurquoise2

Use

AAV

Promoter

CAG

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-5U-Venus-PSD-95-3U

Plasmid

#102949

Purpose

Expresses Venus-PSD-95 in mammalian cells

Depositor

Gary Bassell

Article

Ifrim et al J Neurosci. 2015 May 6;35(18):7116-30. d

Insert

PSD-95 (Dlg4 Mouse)

Tags

Venus Fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSNAP-CaaX Control Plasmid

Plasmid

#101133

Purpose

Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

CaaX Box

Tags

SNAP-tag (SNAP26m)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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FTL

Plasmid

#100750

Purpose

Part of FerriTag expression system. Untagged Ferritin light chain in mammalian expression vector.

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

FTL (FTL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PIF3-mEGFP

Plasmid

#100283

Purpose

Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome interacting factor 3 (PIF3 Mustard Weed)

Tags

mEGFP

Expression

Mammalian

Mutation

N-terminus (1-100 aa) of PIF3 is used

Promoter

CAG promoter

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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EKARet-cyto

Plasmid

#96895

Purpose

FLIM sensor for ERK activation in cytosol

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

FLIM Sensor for ERK Activity in Cytosol

Tags

EGFP and sREAChet

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-IRAlus-Nicn1

Plasmid

#92345

Purpose

Plasmid for expression of GFP gene with a Nicn1 gene derived IR Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Nicn1 gene derived IR Alu element containing 3'UTR (NICN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV p2GM AMPK alpha2hp1 alpha1hp1

Plasmid

#89492

Purpose

shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

2 hairpins against mouse/human AMPKalpha1 and alpha2

Use

Retroviral

Expression

Mammalian

Promoter

U6 (The GFP-Puro is from a PGK promoter; the shRN…

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA

Plasmid

#89459

Purpose

constitutive expression of human CHD7 in mammalian cells

Depositor

Peter Scacheri

Insert

CHD7 (CHD7 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-RLuc8.6-535

Plasmid

#87125

Purpose

Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

Depositor

Sanjiv Sam Gambhir

Article

Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

Insert

Renilla Luciferase

Expression

Mammalian

Mutation

RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST26-C-FLAG

Plasmid

#79275

Purpose

Destination vector containing a C-terminal FLAG tag

Depositor

Rita Shiang

Article

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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