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pAM-PAT-ProGL2 GW

Plasmid

#80689

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Weinl et al Plant Cell. 2005 Jun;17(6):1704-22. Epub

Type

Empty backbone

Expression

Plant

Promoter

ProGL2

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*V106Q)

Plasmid

#125651

Purpose

pCMV-ABEmax(TadA E59A, TadA*V106Q) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* V106Q)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*R47Q)

Plasmid

#125657

Purpose

pCMV-ABEmax(TadA E59A, TadA*R47Q) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* R47Q)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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Cerulean(159-238) in pcDNAI/Amp

Plasmid

#60898

Purpose

This is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

Cerulean(159-238)

Expression

Mammalian

Mutation

Contains N164H substitution

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTUT6

Plasmid

#60043

Purpose

Mammalian expression of FLAG-tagged mouse TUT6

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT6 (Tut1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS DNAJB3

Plasmid

#19552

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3 Mouse)

Tags

His

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-2a-1

Plasmid

#22764

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 2a-1 (mir-2a-1 primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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His8-TEV-PseH

Plasmid

#89725

Purpose

Expresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseH (pseH Campylobacter jejuni)

Tags

2xHis Gln 5xHis and TEV

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSY091

Plasmid

#110068

Purpose

Bacterial expression of L4

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L4

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSY075

Plasmid

#110067

Purpose

Bacterial expression of L2

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L2

Expression

Bacterial

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-13a

Plasmid

#22761

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 13a (mir-13a primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P03

Plasmid

#120014

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHYDuet93

Plasmid

#42303

Depositor

Hideo Iwai

Article

Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

Insert

NpuDnaEdC6

Tags

H6-GB1

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM1-NtermRGS2-GFP-CIBN

Plasmid

#112258

Purpose

Expresses alpha-helix component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

NtermRGS2-GFP-CIBN

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-CICf_W37A

Plasmid

#48186

Purpose

Expresses W37A mutated Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM390

Plasmid

#84202

Purpose

Disturb the expression of miR390 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR390

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM393

Plasmid

#84203

Purpose

Disturb the expression of miR393 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR393

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM156

Plasmid

#84194

Purpose

Disturb the expression of miR156 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR156

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
pGL3-PIM2mut1

Plasmid

#51849

Purpose

promoter region of PIM2 lacking the forkhead binding site #2

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T-1-mMcl-1(S140A)

Plasmid

#25381

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1(S140A) (Mcl1 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

changed serine 140 to alanine

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only