We narrowed to 13,693 results for: crispr cas9

1. pML104-HygMx4-URA3-3

   Plasmid

   #232886

   Purpose

   Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   URA3 gRNA (URA3 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   snR52

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML104-HygMx4-URA3-2

   Plasmid

   #232885

   Purpose

   Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   URA3 gRNA (URA3 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   snR52

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pML107-AVO1

   Plasmid

   #232889

   Purpose

   Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   AVO1 gRNA (AVO1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   snR52

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pML107-YFR054C

   Plasmid

   #232900

   Purpose

   Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   YFR054C gRNA (YFR054C Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pML107-ZIM17

   Plasmid

   #232901

   Purpose

   Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   ZIM17 gRNA (ZIM17 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pML107-SRP14

   Plasmid

   #232896

   Purpose

   Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   SRP14 gRNA (SRP14 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pML107-TLG1

   Plasmid

   #232897

   Purpose

   Plasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   TLG1 gRNA (TLG1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pML107-VHT1

   Plasmid

   #232898

   Purpose

   Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   VHT1 gRNA (VHT1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pML107-ADE13

   Plasmid

   #232887

   Purpose

   Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   ADE13 gRNA (ADE13 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   snR52

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pML107-LAS17

    Plasmid

    #232892

    Purpose

    Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LAS17 gRNA (LAS17 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    snR52

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pML104-LEU2-2

    Plasmid

    #232883

    Purpose

    Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LEU2 gRNA (LEU2 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    snR52

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pML107-INO80

    Plasmid

    #232890

    Purpose

    Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    INO80 gRNA (INO80 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    snR52

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCG-scr

    Plasmid

    #229847

    Purpose

    Expresses wild-type Cas9 and scrambled gRNA.

    Depositor

    Natsuko Chiba

    Insert

    guide RNA with scrambled sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. HCP1

    Plasmid

    #166103

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.

    Depositor

    Matthias Heinemann

    Insert

    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pdpy-30-miniCAFE

    Plasmid

    #170115

    Purpose

    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    Dpy-30

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PL-5LTR-RGR(DMD​#​1​)-AmCyan-A

    Plasmid

    #138482

    Purpose

    Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Ribozyme-flanked gRNA and AmCyan
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. plenti-Lb-Cas12a-2xNLS

    Plasmid

    #155046

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. plenti-As-Cas12a-2xNLS

    Plasmid

    #155047

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    AsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. B2M Bulldozer (gRNA crB2M_13)

    Plasmid

    #84381

    Purpose

    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells

    Depositor

    Chad Cowan

    Article

    Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.

    Insert

    B2M Bulldozer crB2M_13 gRNA
    

    Expression

    Mammalian

    Promoter

    hU6 promoter

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97308

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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