We narrowed to 17,101 results for: Por

1. YjdC-R64A_pET-14b

   Plasmid

   #233263

   Purpose

   Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

   Depositor

   Mohan Babu, Andrew Emili

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   YjdC (yjdC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 64…

   Promoter

   T7 promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. HemH-R212A_pET-14b

   Plasmid

   #233262

   Purpose

   Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

   Depositor

   Mohan Babu, Andrew Emili

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   HemH (hemH )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 21…

   Promoter

   T7 promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVtrunc F-tractinN9-39-msfGFPΔN7

   Plasmid

   #231552

   Purpose

   Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   F-tractin (Itpka Rat)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMVtrunc LifeactΔC4-msfGFPΔN7

   Plasmid

   #231555

   Purpose

   Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Lifeact (ABP140 Budding Yeast)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVtrunc msfGFPΔC9-LifeactΔN2

   Plasmid

   #231556

   Purpose

   Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Lifeact (ABP140 Budding Yeast)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONOR-MARINA

   Plasmid

   #223328

   Purpose

   DONOR vector for genomic integration of MARINA voltage sensitive indicator gene from Platisa et al., 2017 (10.1021/acschemneuro.6b00234).

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Inserts

   MARINA
   KIR2.1 channel Golgi-to-plasma membrane trafficking signal
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mCherry and super-ecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

   Plasmid

   #228787

   Purpose

   EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   EGFP***
   TEV protease
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFBOH-MHL.RING1B

   Plasmid

   #224712

   Purpose

   Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   RING1b (RNF2 Human)
   

   Use

   Tags

   6x histidine tag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available Since

   Oct. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pFBOH-MHL.BMI1

   Plasmid

   #224711

   Purpose

   Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   BMI1 (BMI1 Human)
   

   Use

   Tags

   6x histidine tag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-Tet-O-EYA2-Puro

    Plasmid

    #221669

    Purpose

    Expresses EYA2 in mammalian cells

    Depositor

    Vanessa Hall

    Article

    Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

    Insert

    EYA2 (EYA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-Tet-O-Foxp1A-Puro

    Plasmid

    #221665

    Purpose

    Expresses Foxp1A in mammalian cells

    Depositor

    Vanessa Hall

    Article

    Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

    Insert

    Foxp1A (Foxp1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-CASK

    Plasmid

    #221427

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    CASK (CASK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNES-SET-EMD-EGFP (C-NES-SET-EMD)

    Plasmid

    #217770

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    SET (SET Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EMD domain (aa 70-226) plus nuclear export sequen…

    Promoter

    CMV

    Available Since

    June 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188743

    Purpose

    Donor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188744

    Purpose

    Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188745

    Purpose

    Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    nonTATAchr21

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188746

    Purpose

    Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    nonTATAchrX

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. shTRIP13-2-seed

    Plasmid

    #184535

    Purpose

    shRNA expression vector

    Depositor

    Andrew Hong

    Article

    Mittal et al Commun Biol. 2024 Apr 8;7(1):426. doi: 1

    Insert

    TRIP13 (TRIP13 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. shTRIP13-1

    Plasmid

    #184536

    Purpose

    shRNA expression vector

    Depositor

    Andrew Hong

    Article

    Mittal et al Commun Biol. 2024 Apr 8;7(1):426. doi: 1

    Insert

    TRIP13 (TRIP13 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. shTRIP13-2

    Plasmid

    #184537

    Purpose

    shRNA expression vector

    Depositor

    Andrew Hong

    Article

    Mittal et al Commun Biol. 2024 Apr 8;7(1):426. doi: 1

    Insert

    TRIP13 (TRIP13 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge