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pMYT002

Plasmid

#180655

Purpose

Encodes pLacD as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pLacD

Use

Synthetic Biology

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT002

Plasmid

#180655

Purpose

Encodes pLacD as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pLacD

Use

Synthetic Biology

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMA5096

Plasmid

#184850

Purpose

General purpose retroviral cloning vector, hygromycin resistance

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMA5096

Plasmid

#184850

Purpose

General purpose retroviral cloning vector, hygromycin resistance

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT22i2Shh24G4FER3(#1041)

Plasmid

#184072

Purpose

cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Insert

Gal4bdFF-ERT2

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Shha2.4

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pT22i2Shh24G4FER3(#1041)

Plasmid

#184072

Purpose

cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Insert

Gal4bdFF-ERT2

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Shha2.4

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pT2iaTubG4FER3(#940)

Plasmid

#184071

Purpose

cyclofen-inducible GAL4 activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Insert

Gal4bdFF-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Gfish-a1Tub

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT2iaTubG4FER3(#940)

Plasmid

#184071

Purpose

cyclofen-inducible GAL4 activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Insert

Gal4bdFF-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Gfish-a1Tub

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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Akaluc VLP

Plasmid

#182212

Purpose

YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).

Depositor

Claudia Vickers

Inserts

VP2C-Akaluc
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology; Tem imaging

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Akaluc VLP

Plasmid

#182212

Purpose

YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).

Depositor

Claudia Vickers

Inserts

VP2C-Akaluc
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology; Tem imaging

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a_SUS1 5'SS

Plasmid

#100991

Purpose

Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

GGTAtGT->tGTAtGa 5'SS mutant

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a_No_BP-pUC18

Plasmid

#100992

Purpose

Contains model Rp51a with a TACTAAC->GTTAGTG BP mutataion

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

TACTAAC->GTTAGTG BP mutataion

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a_SUS1 5'SS

Plasmid

#100991

Purpose

Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

GGTAtGT->tGTAtGa 5'SS mutant

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a_No_BP-pUC18

Plasmid

#100992

Purpose

Contains model Rp51a with a TACTAAC->GTTAGTG BP mutataion

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

TACTAAC->GTTAGTG BP mutataion

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEDJ508

Plasmid

#177275

Purpose

ADH1t:T7p:cgRNA_HIS3_stop:tZ

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

ADH1t:T7p:cgRNA_HIS3_stop:tZ

Expression

Bacterial and Yeast

Promoter

N/A

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEDJ508

Plasmid

#177275

Purpose

ADH1t:T7p:cgRNA_HIS3_stop:tZ

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

ADH1t:T7p:cgRNA_HIS3_stop:tZ

Expression

Bacterial and Yeast

Promoter

N/A

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET_His-rsCaMPARI-mRuby3

Plasmid

#120804

Purpose

Expresses rsCaMPARI in bacterial cells

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Tags

His-Xpress and mRuby3

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET_His-rsCaMPARI-mRuby3

Plasmid

#120804

Purpose

Expresses rsCaMPARI in bacterial cells

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Tags

His-Xpress and mRuby3

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pNR1D2.1.0-gDNA

Plasmid

#112410

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR1D2

Depositor

Kevin White

Insert

NR1D2 (NR1D2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pNR1D2.1.0-gDNA

Plasmid

#112410

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR1D2

Depositor

Kevin White

Insert

NR1D2 (NR1D2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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