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We narrowed to 4,744 results for: NAP

Showing: 1001 - 1020 of 4744 results

  4. pFsynW SYT1-PGM reporter

    Plasmid
    #197278
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A
    Promoter
    hSyn (human synapsin I promoter)
    Available Since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197278-plasmid-map-sequence-id-389096

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  6. pF(UG) hSyn NS34a-msGFP-A'Q-mito

    Plasmid
    #158766
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available Since
    Availability
    Academic Institutions and Nonprofits only
    158766-plasmid-map-sequence-id-313130

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  7. pF(UG) hSyn NS34a-msGFP-'Q-mito

    Plasmid
    #158767
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available Since
    Availability
    Academic Institutions and Nonprofits only
    158767-plasmid-map-sequence-id-313145

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  14. pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3

    Plasmid
    #165430
    Purpose
    Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    FHS-paCaMKII(SD)
    Use
    AAV
    Tags
    FHS(Flag, HisX6, Strep) tag
    Expression
    Mammalian
    Mutation
    C450A, L514K, G528A, L531E, N538E: Light-insensit…
    Promoter
    CaMKII0.4
    Available Since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165430-plasmid-map-sequence-id-325945

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  16. CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA

    Plasmid
    #165435
    Purpose
    Expresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA
    Use
    Tags
    ShadowY, Clover(T153M/F223R)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165435-plasmid-map-sequence-id-325982

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Showing: 1001 - 1020 of 4744 results