Addgene

We narrowed to 2,166 results for: ada.2

Showing: 1001 - 1020 of 2166 results

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pAc5.1B-EGFP-DmNot2-dsRNAres_Q

Plasmid

#147341

Purpose

Insect Expression of DmNot2-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot2-dsRNAres (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAM90

Plasmid

#226682

Purpose

Rpn9S111TAG lid (Rpn5, MBP-HRV-Rpn6, Rpn8, Rpn9S111TAG, Rpn11)

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Inserts

Rpn5 (RPN5 Budding Yeast)
Rpn6 (RPN6 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)
Rpn9 (RPN9 Budding Yeast)
Rpn11 (RPN11 Budding Yeast)

Use

Tags

MBP-HRV

Expression

Bacterial

Mutation

S111TAG

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pαH-S-RRAR-OMICRON

Plasmid

#180424

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intact

Depositor

Priyamvada Acharya

Article

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

Spike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL

Plasmid

#111390

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
ChETA-mRuby2

Use

AAV

Tags

Myc and mRuby2

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_CapChR2-GFP

Plasmid

#224357

Purpose

Expresses CapChR2-GFP in mammalian cells

Depositor

Adam Cohen

Article

Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

Insert

CapChR2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-OMICRON

Plasmid

#180423

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS

Depositor

Priyamvada Acharya

Article

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano65/pcDNA3

Plasmid

#51965

Purpose

mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano65

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
Yellow Cameleon-Nano15/pcDNA3

Plasmid

#51961

Purpose

mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano15

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
Yellow Cameleon-Nano30/pcDNA3

Plasmid

#51963

Purpose

mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive Ca2+ indicator with Kd = 30 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano30

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pW229-lenti-sg2-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189945

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg2-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW318-lenti-sg2-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189948

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg2-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW402-lenti-sg2-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189951

Purpose

Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg2-Itgb1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano15/pBig

Plasmid

#51962

Purpose

Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMK198 (GAL-OsTIR1)

Plasmid

#140655

Purpose

GAL-OsTIR1(WT)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-KLF5

Plasmid

#40918

Depositor

Jin-Tang Dong

Article

Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

Insert

Kruppel-like factor 5 (KLF5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-KLF5-FLAG

Plasmid

#40917

Depositor

Jin-Tang Dong

Article

Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

Insert

Kruppel-like factor 5 (KLF5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
Yellow Cameleon-Nano140/pcDNA3

Plasmid

#51966

Purpose

mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano140

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-KLF5-his

Plasmid

#40904

Depositor

Jin-Tang Dong

Article

Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

Insert

Kruppel-like factor 5 (KLF5 Human)

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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sABE v3.22 Lenti_1

Plasmid

#209555

Purpose

pCMV-SV40NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano50/pcDNA3

Plasmid

#51964

Purpose

mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano50

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge