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Showing: 1001 - 1020 of 2791 results

  1. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid
    #112713
    Purpose
    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    Rngtt (Rngtt Mouse)
    Use
    Tags
    BirA* (BirA R118G), HIV Rev nuclear localization …
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    112713-plasmid-map-sequence-id-217067

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  8. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid
    #140980
    Purpose
    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasS-RLuc8
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140980-plasmid-map-sequence-id-300680

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  10. pcDNA3.1(+)GαsS-246-Nluc

    Plasmid
    #207157
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAS (GNAS Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(246)N
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207157-plasmid-map-sequence-id-410282

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  11. pcDNA3.1(+)GαsL-260-NLuc

    Plasmid
    #207158
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAS (GNAS Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(260)N
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207158-plasmid-map-sequence-id-410280

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  19. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid
    #114111
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100
    Insert
    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114111-plasmid-map-sequence-id-252509

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Showing: 1001 - 1020 of 2791 results