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FKBP-GFP-BAF

Plasmid

#222414

Purpose

To express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

BAF (BANF1 Human)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Ideonella-bGSDM-WELQ

Plasmid

#222098

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Ideonella-bGSDM-WELQ ORF

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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AA335

Plasmid

#216069

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; HygroR_v1.1; Stop

Use

Fragment

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast CG1358

Plasmid

#218541

Purpose

Drosophila CG1358 cDNA (human codon optimized)

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CG1358 (HisT Fly)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg2

Plasmid

#218531

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg1

Plasmid

#218530

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA020

Plasmid

#216004

Purpose

Fragmid fragment: (Cas protein) binds PP7 stem loops

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

PP7 Core Protein (PCP)_v1.1

Use

CRISPR; Fragment

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA366

Plasmid

#215970

Purpose

Fragmid fragment: (Pol 2 promoter) Gal4-induced expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

5xUAS_v1; 5xUAS_v1; 5xUAS_v1; 5xUAS_v1; Hsp70_v2; Mhc_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA436

Plasmid

#216035

Purpose

Fragmid fragment: (Cas protein) binds MS2 stem loops

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

MS2_v1.1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA440

Plasmid

#216036

Purpose

Fragmid fragment: (Cas protein) binds to TRE in absence of doxycycline

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

TetR_v1.1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA201

Plasmid

#216026

Purpose

Fragmid fragment: (Cas protein) firefly luciferase

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Fluc_v1.1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA033

Plasmid

#216013

Purpose

Fragmid fragment: (Cas protein) deactivated Cas

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

dCas9-NG_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA333

Plasmid

#216067

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; PuroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; PuroR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA363

Plasmid

#215955

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

dU6:3_v1; BsmBI_v14; BsmBI_v1; trRNA_v1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA271

Plasmid

#216074

Purpose

Fragmid fragment: (CROPseq) guide expression; UMI-compatible

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]; 3'LTR; bGHpolyA_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA156

Plasmid

#216022

Purpose

Fragmid fragment: (Cas protein) Nanobody binds ALFA tag

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NbALFA_v1.1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA391

Plasmid

#216033

Purpose

Fragmid fragment: (Cas protein) reverse TetR; binds to TRE in presence of doxycycline

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rTetR_v1.1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA303

Plasmid

#216071

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v6; trRNA_v1 [Nme2]; terminator_v1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA349

Plasmid

#216032

Purpose

Fragmid fragment: (Cas protein) Renilla luciferase

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Rluc_v1.1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA035

Plasmid

#216015

Purpose

Fragmid fragment: (Cas protein) deactivated Cas

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

dCas9-SpRY_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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