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Showing: 10201 - 10220 of 164851 results

  4. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  12. INCTbiosyn-pEF-GFP(rc)9

    Plasmid
    #127508
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 9 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 9 attachment sites.
    Expression
    Mammalian
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127508-plasmid-map-sequence-id-250750

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  13. INCTbiosyn-pEF-GFP(rc)13

    Plasmid
    #127509
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
    Expression
    Mammalian
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127509-plasmid-map-sequence-id-250751

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  14. INCTbiosyn-pEF-GFP(rc)2

    Plasmid
    #127504
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
    Expression
    Mammalian
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127504-plasmid-map-sequence-id-250743

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Showing: 10201 - 10220 of 164851 results