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pcDNA-ER-ZapCY1

Plasmid

#36321

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ER-ZapCY1 high affinity Zn(II) sensor

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV_2pabPAC

Plasmid

#158969

Purpose

2P-activatable bPAC for AAV

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Use

AAV

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO3

Plasmid

#53143

Purpose

E.coli expression of untagged mature human SUMO3 (amino acids 1-93)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO3 (SUMO2 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-93)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM mouse dbx-1 [TJ#11]

Plasmid

#16269

Depositor

Thomas Jessell

Article

Pierani et al Cell. 1999 Jun 25. 97(7):903-15.

Insert

dbx1 3'UTR (Dbx1 Mouse)

Expression

Bacterial

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pCas9CyMutL

Plasmid

#166981

Purpose

Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Inserts

MutL (mutL )
CymR

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

E32K

Promoter

pCymRC and pLacI

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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E.coli RaPID plasmid

Plasmid

#107251

Purpose

Expresses the E.coli RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

E.coli BirA*

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-mGFP-Sec23B

Plasmid

#66598

Purpose

Expresses Sec23B as a fusion with mGFP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec23B (SEC23B Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pORF1-Flag

Plasmid

#190565

Purpose

Mammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.

Depositor

Anthony Furano

Article

Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

ORF1 (from L1.3)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-EGFP-Ecadherin

Plasmid

#65288

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pPI356

Plasmid

#207449

Purpose

Extrachromosomal expression vector PkgE-PH-GFP and PTEN-mCherry

Depositor

Rob Kay

Article

Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

Inserts

PkgE
PTEN

Use

Unspecified

Tags

GFP and mCherry

Mutation

PH domain only

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ141D-peg

Plasmid

#141081

Purpose

Gateway compatible prime editing gRNA empty backbone vector

Depositor

Yiping Qi

Article

Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSR prdm3 (PRDM16 shRNA)

Plasmid

#15505

Depositor

Bruce Spiegelman

Article

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 shRNA (Prdm16 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
mRuby2-pBAD

Plasmid

#54771

Purpose

Localization: Protein Expression Vector , Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

6xHis and mRuby2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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BSA1_His

Plasmid

#202766

Purpose

bovine serum albumin design BSA1, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2-Huc-Positron-ST

Plasmid

#129265

Purpose

pan neuronal expression of soma-localized Positron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Mutation

D92N, E199V

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMK782 Septin6-GFP

Plasmid

#38296

Depositor

Matthew Krummel

Article

Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

Insert

Septin 6 (Septin6 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

22 amino acid linker sequence (added by PCR)

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-mouse-Caspase-1

Plasmid

#183361

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (Casp1 Mouse)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5A-V5

Plasmid

#35930

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-mCherry-Ecadherin

Plasmid

#65289

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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