We narrowed to 28,641 results for: Tat

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plko-tet-on-puromycin-shTRX1-259

Plasmid

#110920

Purpose

To knockdown Thioredoxin-1 following doxycycline addition

Depositor

Priyamvada Rai

Article

Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

Insert

TRX1 (TXN Human)

Use

Lentiviral

Promoter

unknown

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-hDDB2-K244E-V5

Plasmid

#167471

Purpose

ectopic expression of hDDB2 in mammalian cells

Depositor

Steven Artandi

Article

Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

Insert

DDB2 (DDB2 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

K244E

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pUDR217

Plasmid

#113872

Purpose

expression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA9-MPH2-3 sgRNA10-MAL11

Use

CRISPR

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G12-E

Plasmid

#25757

Purpose

Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-PLXNB2-decto

Plasmid

#86238

Purpose

Lentivirus for expression of human Plexin-B2 with deletion of ectodomain

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PLXNB2 with deletion of extracellular domain

Promoter

hPGK

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-PLXNB2-dVTDL

Plasmid

#86239

Purpose

Lentivirus for expression of human Plexin-B2 with deletion of C-terminal VTDL aminoacids

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PLXNB2 with deletion of last 4 aminoacids

Promoter

hPGK

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-Idax DBM

Plasmid

#68554

Purpose

This plasmid expresses full length IDAX containing a DNA binding mutation.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

IDAX-DBM (Cxxc4 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

T162A, H164A and Q165A mutations

Promoter

EF1a

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-Cx43ML-N1

Plasmid

#49860

Purpose

Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

All internal Methionines mutated to Leucine. No s…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 P5-6D

Plasmid

#51254

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 923,956 mutated to Asp

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO/SH/GW-Mst2

Plasmid

#84300

Purpose

Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst2. Used in conjunction with Flp-In cells to generate single copy transgene.

Depositor

Jonathan Chernoff

Article

Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

Insert

Mst2 (STK3 Human)

Tags

HA, SBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-hDIXDC1 S592A

Plasmid

#61223

Purpose

Mammalian expression vector encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 592 to Alanine

Promoter

CMV

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
FUW TetO NET1A-V5 WT

Plasmid

#69828

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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TRPM7 gRNA (BRDN0001146152)

Plasmid

#76111

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM7 (TRPM7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)

Plasmid

#119312

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-500

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 1-376)

Plasmid

#119311

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-376 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-376

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)

Plasmid

#119316

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 481-852

Promoter

CMV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CCR5(R5gp120-Hi)

Plasmid

#98954

Purpose

Mammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-Hi

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

R5gp120-Hi variant: P8L, I9E, I12M, S17E, P19D, Q…

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrGAP R1090A

Plasmid

#38176

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)

Tags

Xpress

Expression

Mammalian

Mutation

aa 1004-1271 with R1090A point mutation

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pXPR_BRD003-sgRBFOX1-2083

Plasmid

#115451

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgRBFOX1-2083 (RBFOX1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSN67

Plasmid

#100124

Purpose

expresses putative KLF4 DBD with a 6xHis tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Insert corresponds to the DBD of KLF4

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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