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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 37,489 results for: crispr

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Showing: 10261 - 10280 of 37489 results

  8. pADH139

    Plasmid
    #90987
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.
    Depositor
    Aaron Hernday
    Publication
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, C, mal LEU2 2 of 2
    Use
    CRISPR
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90987-plasmid-map-sequence-id-180520

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  11. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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  14. pX330-PITCh-TOMM20

    Plasmid
    #207789
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Dec. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    207789-plasmid-map-sequence-id-455792

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  16. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid
    #74368
    Purpose
    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.
    Depositor
    Eric Klavins
    Publication
    Gander et al bioRxiv 041871
    Insert
    yeGFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    pGRR-r11-r10
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74368-plasmid-map-sequence-id-141319

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  19. pY112 (pcDNA3.1-huSaCpf1)

    Plasmid
    #92269
    Purpose
    Expression of humanized SaCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huSaCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92269-plasmid-map-sequence-id-181496

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  20. pY114 (pcDNA3.1-huPgCpf1)

    Plasmid
    #92273
    Purpose
    Expression of humanized PgCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huPgCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92273-plasmid-map-sequence-id-181493

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Showing: 10261 - 10280 of 37489 results