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pCAG-p21_T145A

Plasmid

#40203

Depositor

Philipp Mergenthaler

Article

Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

Insert

p21 (CDKN1A Human)

Expression

Mammalian

Mutation

T145A

Promoter

CAG

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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GST-Cav (135-178)

Plasmid

#22448

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin 1

Tags

GST

Expression

Bacterial

Mutation

aa 135-178

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only
GST-Cav (61-101)

Plasmid

#22447

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1 (61-101)

Tags

GST

Expression

Bacterial

Available Since

Jan. 26, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-6P1-Ataxin3Q22-UIM*

Plasmid

#22114

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Tags

GST

Expression

Bacterial

Mutation

UIM 1, 2, 3 SA-DG

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-V660N

Plasmid

#12292

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y and V660N; signal peptide (aa1-23) re…

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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AiP15745: pAAV-AiE0452h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5745)

Plasmid

#230880

Purpose

AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mScarlet

Use

AAV

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15043 - pAAV-AiE1419m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5043)

Plasmid

#224189

Purpose

AiE1419m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14361: pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4361)

Plasmid

#214857

Purpose

Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

jGCaMP8s

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496)

Plasmid

#214858

Purpose

Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13450: pAAV-AiE0140h-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN3450)

Plasmid

#214845

Purpose

Expression of iCre in striatal Pvalb neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408)

Plasmid

#191728

Purpose

AI plasmid ALIAS: AiP12408. Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12609 - pAAV-AiE0779m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2609)

Plasmid

#191705

Purpose

AI plasmid ALIAS: AiP12609. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-OleTLA

Plasmid

#251243

Purpose

Expression of CYP152-OleTLA

Depositor

Pimchai Chaiyen

Article

Phaisan et al J Biol Chem. 2025 Jul;301(7):110397. doi

Insert

OleTLA

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

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pSWITCHER-TF1-dsRED

Plasmid

#213043

Purpose

SWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

SWITCHER-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

cBH

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-LbCpf1-crF1

Plasmid

#213046

Purpose

Guide RNA expression vector with the synthetic crRNA crF1 targeting the SWITCHER construct with the target site TF1

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crF1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-LbCpf1-crR2

Plasmid

#213047

Purpose

Guide RNA expression vector with the synthetic crRNA crR2 targeting the SWITCHER construct with the target site TR2

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crR2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLoxP-EGFP-crRNA-entry

Plasmid

#213048

Purpose

SWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

LoxP-EGFP-MALAT1-triplex

Use

CRISPR

Expression

Mammalian

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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L1p1_min35S_Luciferase

Plasmid

#231035

Purpose

Cloning vector to clone promoter fragment in front of minimal p35S:Luciferase:tNOS

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Minimal p35S:Luciferase:tNOS

Use

Luciferase

Expression

Bacterial

Promoter

minimal p35S

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM_ΔndhD2.km

Plasmid

#185532

Purpose

a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus

Depositor

Paula Tamagnini

Article

Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

Insert

nptII gene flanked by ndhD2 upstream and downstream regions

Use

Synthetic Biology

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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