We narrowed to 59,942 results for: %s

1. pRSETC-PITPbeta (splice variant 1) K61A

   Plasmid

   #58271

   Purpose

   bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

   Depositor

   Shamshad Cockcroft

   Article

   Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

   Insert

   PITP beta (PITPNB Human)
   

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   K61A (abolishes PI binding)

   Promoter

   T7

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCI Flag Smad3 NL* EPSM

   Plasmid

   #27005

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   aa 1-203; T179V

   Available Since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCI Flag Smad3 NL* WT

   Plasmid

   #27042

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   aa 1-203

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pQCXIH-Flag Smad3 AAGY

   Plasmid

   #27027

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   P181A P182A

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCI Flag Smad3 NL AAGY

   Plasmid

   #27041

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   aa 1-226; P181A P182A

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pGEX6-Smad3 EPSM

   Plasmid

   #27222

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T179V S204A S208A S213A

   Available Since

   Dec. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pGEX6-Smad3 AAGY

   Plasmid

   #27013

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   P181A P182A

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3 PARL-FLAG-CT S65A

   Plasmid

   #13618

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   PARL (PARL, PARL Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 65 to Alanine

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3 PARL-FLAG-CT T69A

   Plasmid

   #13619

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Thr 69 to Ala

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3 PARL-FLAG-CT S65D

    Plasmid

    #13621

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 65 to Asp

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3 PARL-FLAG-CT T69D

    Plasmid

    #13622

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Thr 69 to Asp

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3 PARL-FLAG-CT S70D

    Plasmid

    #13623

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 70 to Asp

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pAV10

    Plasmid

    #63213

    Purpose

    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET41a iGlum

    Plasmid

    #119829

    Purpose

    Production of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGlum
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    R24K

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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15. HM3C NaVSp1 pET24b

    Plasmid

    #128088

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

    Depositor

    Dan Minor

    Article

    Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

    Insert

    NaVSp1p
    

    Tags

    3C Cleavage, His Tag, and MBP

    Expression

    Bacterial

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSW031

    Plasmid

    #231144

    Purpose

    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

    Depositor

    Philip Cole

    Article

    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

    Insert

    S. aureus Sortase A mutant
    

    Tags

    Nostoc punctiform split intein C-terminal fragmen…

    Expression

    Bacterial

    Mutation

    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

    Promoter

    T7

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-PHGDH

    Plasmid

    #74113

    Purpose

    Expression of N-terminal His-tagged

    Depositor

    Lewis Cantley

    Article

    Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    PHGDH (PHGDH Human)
    

    Tags

    His-tagged

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-CRT-Fc FL

    Plasmid

    #221853

    Purpose

    Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    calreticulin (CALR Human)
    

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST17-ALDH1A1

    Plasmid

    #189749

    Purpose

    The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A1 (ALDH1A1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. R620-M06-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417N/E484K/N501Y

    Plasmid

    #170192

    Purpose

    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S SARS-CoV-2)
    

    Tags

    3C-His8-SBP

    Expression

    Mammalian

    Mutation

    K417N, E484K, N501Y

    Promoter

    CMV51

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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