We narrowed to 23,036 results for: his

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pET-PLS-KP6-A66S_rpoA-U

Plasmid

#190969

Purpose

Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP3_rpoA-U

Plasmid

#190960

Purpose

Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-E67A_rpoA-U

Plasmid

#190973

Purpose

Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Centrin3

Plasmid

#136807

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 7

Plasmid

#36105

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 7 (GNG7 Bovine)

Tags

YFP

Expression

Mammalian

Mutation

HIs tag between YFP and insert

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
CKAP2 pHAT

Plasmid

#175946

Purpose

Bacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Susanne Bechstedt

Article

McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

Insert

CKAP2 (Ckap2 Mouse)

Tags

6-His and StrepTagII

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 FYN

Plasmid

#67106

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

FYN (FYN Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CREBZF

Plasmid

#67070

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CREBZF (CREBZF Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
HOXD12 (murine) pRSETa

Plasmid

#8554

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXD12 (Hoxd12 Mouse)

Tags

HIS and XPress

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pmCellFree_KA2_ZIKV NS2A

Plasmid

#145363

Purpose

Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Tags

His and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJK2067

Plasmid

#171845

Purpose

C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

Depositor

Judith Kimble

Article

Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

Insert

his-58, eGFP, mCherry

Tags

N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

Expression

Worm

Mutation

boxB wild type and hairpin mutant

Promoter

mex-5 promoter

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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K339CLM G234A/215/347

Plasmid

#24449

Depositor

Ron Vale

Article

Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

Insert

Kinesin (KIF5B Human)

Tags

His

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S, G234A/E215C/E…

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Chicken, Synthetic)

Tags

GFP-His8

Expression

Yeast

Promoter

GAL1

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
HOXA9 (murine) FLAG pET

Plasmid

#8494

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Tags

FLAG and His

Expression

Bacterial

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 nu

Plasmid

#44940

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 nu (GRF7 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CSNK2A1

Plasmid

#67085

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
D92_Ld_360170

Plasmid

#135905

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFA-sfGFP

Plasmid

#162689

Purpose

pFA vector control expressing sfGFP

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

sfGFP

Tags

C terminal 6x His tag

Expression

Bacterial

Promoter

PL tet promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LacD.1_WT_pET21a

Plasmid

#29789

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))

Tags

His

Expression

Bacterial

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only