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pK3FS-PYK1

Plasmid

#85777

Purpose

N-ICE plasmid pK3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAav-MCS-PQS2-3xHA

Plasmid

#84917

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

LOX-PGK-NEO-LOX

Use

AAV

Tags

PQS2 3xHA

Promoter

mPGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE76

Plasmid

#80578

Purpose

recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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PmarA-cfp

Plasmid

#79867

Purpose

Degradation tagged CFP fluorescent reporter for MarA

Depositor

Mary Dunlop

Article

El_Meouche et al Sci Rep. 2016 Jan 13;6:19538. doi: 10.10

Insert

A modified marRAB promoter upstream of a degradation tagged cfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

modified marRAB promoter with inactivated MarR bi…

Available Since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJK370

Plasmid

#73595

Purpose

Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

Depositor

T. J. Kappock

Inserts

delta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 2)

Tags

FLAG tag and c-myc tag

Expression

Yeast

Mutation

Silent mutation in Arg299 codon.

Promoter

GAL1 and GAL10

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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ptdEos-CLC

Plasmid

#38009

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Inserts

Clathrin, light polypeptide (LCa) (Clta Mouse)
Eos FP, tandem dimer

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pET41s-GST-ATF6a-AD

Plasmid

#64774

Purpose

bacterial expression of GST-tagged ATF6 (1-150)

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

ATF6a-AD (1-150) (ATF6 Human)

Tags

GST

Expression

Mammalian

Mutation

C-term deletion

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

Plasmid

#55216

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pHL 1405

Plasmid

#53010

Purpose

pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
DsrA sRNA
hfq
sodB

Use

Synthetic Biology

Tags

gfp

Expression

Bacterial

Mutation

contains -56 to +141 relative to start codon

Promoter

pCon, pLlacO-1, and pLtetO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
psbA2-Ptrc-PHLS (d)

Plasmid

#52309

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrR1090A-HisB

Plasmid

#32507

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR )

Tags

HISB

Expression

Mammalian

Mutation

R 1090 to A

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Rhoc-wt_3'UTR

Plasmid

#31884

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

Rhoc wild-type 3'UTR (RHOC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
GST-GW1D10(aa566-1343)

Plasmid

#21516

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GST

Expression

Mammalian

Mutation

contain aa566-1343

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
GST-TNR(aa1-204)

Plasmid

#21514

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GST

Expression

Mammalian

Mutation

contain aa1-204

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pBS/U6/hTID1

Plasmid

#13745

Depositor

Karl Munger

Article

Edwards et al Oncogene. 2004 Nov 4. 23(52):8419-31.

Insert

hTid1 siRNA (DNAJA3 Human)

Use

RNAi

Mutation

Inverted repeat of 21 bp with 6 nuc. spacer.

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
piHGpd/G170D

Plasmid

#1113

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 170 changed to aspartic acid; …

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-dCas12a-A3A

Plasmid

#193638

Purpose

Expression of base editor dCas12a-A3A in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3B

Plasmid

#193642

Purpose

Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3B, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3G

Plasmid

#193643

Purpose

Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3G, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag6-iCAM-1

Plasmid

#205210

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG6

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag6 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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