We narrowed to 10,808 results for: T7
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Plasmid#164639PurposeBacterial expression plasmid for His6-MEK1C218DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
TagsHis6-tagExpressionBacterialMutationG(-19)F/S218C/C277S/C376SPromoterT7Available SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0709:Dendra
Plasmid#159077PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0709
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialPromoterpmycTetOAvailable SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJ203
Plasmid#162678PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)DepositorInsertPutative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
TagsHisExpressionBacterialMutationdeltaF2-A75; codon optimized for expression in E.…PromoterT7/lacAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJ1
Plasmid#138379PurposepEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZDepositorTagsHis10ExpressionBacterialMutationClamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…PromoterT7Available SinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet-ECHB(35-475)
Plasmid#96890Purposeexpresses His-tagged mouse ECHB (35-475) in E.coli cellsDepositorInsertTrifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)
TagsHis6 tagExpressionBacterialMutationdeleted amino acid 1-34PromoterT7Available SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET45b-tsr(B/X) (6X-His-Dm-Cofilin)
Plasmid#128278PurposeExpresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell cultureDepositorInserttwinstar (tsr) (tsr Fly)
Tags6X Histidine tagExpressionBacterialMutationMet1 changed to Pro1; Cys77 silent basepair mutat…PromoterT7Available SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-ECHA(37-763)-FLAG (no His-tag)
Plasmid#96887Purposeexpresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)DepositorInsertTrifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)
TagsFLAG-taggedExpressionBacterialMutationdeleted amino acid 1-36PromoterT7Available SinceJuly 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
MK01_HUMAN_D0
Plasmid#79713PurposeThis plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dL5-Her1
Plasmid#73215PurposeBacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)DepositorInsertEGFR (EGFR Schistosoma japonicum, Synthetic, Human)
Tags10XHis-GST-HRV and The dL5 FAP and Her1 affibody …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJTB126
Plasmid#248910Purposeexpresses the yeast analog-sensitive Smk1-Q120A and Ssp2 fused to glutathione-S-transferase in bacteriaDepositorInsertsSMK1 Mitogen-activated Protein Kinase -analog-sensitive form (SMK1 Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes from S288C form, Budding Yeast)
Ssp2 Activator of the Smk1 MAPK (SSP2 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form)
Tagsglutathione-S-transferaseExpressionBacterialMutationcodon 120 has been changed from Q to A (Smk1-Q120…PromoterT7Available SinceJan. 16, 2026AvailabilityAcademic Institutions and Nonprofits only -
*MtbCM-HiBiT-His
Plasmid#228028PurposeExpresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal HiBiT sequence and His tagDepositorInsertRv1885c Chorismate Mutase (Rv1885c Mycobacterium tuberculosis H37Rv)
Tags10xHis-tag with thrombin site and HiBiT TagExpressionBacterialPromoterT7 PromotorAvailable SinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
11_T7-SP-mScarlet3-GPI
Plasmid#229806PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment siteDepositorInsertSP-mScarlet3-GPI
UseIn vitro transcriptionMutationT7 promoterAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-Muc1 mAb
Plasmid#214954PurposeBacterial expression of anti-Muc1 (human) mAb light and heavy chain VNp fusions.DepositorTags6xHis and VNpExpressionBacterialPromoterT7Available SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a HsDHFR-bio-10His
Plasmid#193883PurposeExpresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequenceDepositorInserthuman dihydrofolate reductase (DHFR) (DHFR Human)
TagsHis tag and biotinylation sequenceExpressionBacterialMutationCodon optimizedPromoterT7Available SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en1dFnCas9GFP
Plasmid#201950PurposeExpression of en1dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en1dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1369R on FnCas9PromoterT7Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en15dFnCas9GFP
Plasmid#201951PurposeExpression of en15dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en15dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1603H on FnCas9PromoterT7Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINOH11-A10
Plasmid#184278PurposeExpresses nanobody H11-A10 in E. coliDepositorInsertNanobody H11-A10
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11-B5
Plasmid#184279PurposeExpresses nanobody H11-B5 in E. coliDepositorInsertNanobody H11-B5
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_11-H6
Plasmid#184280PurposeExpresses nanobody H11-H6 in E. coliDepositorInsertNanobody H11-H6
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINH11-H4 Q98R_H100E
Plasmid#184281PurposeExpresses nanobody H11-H4 Q98R_H100E in E. coliDepositorInsertNanobody H11-H4 Q98R_H100E
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only