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pCN34e-kimA-yfp

Plasmid

#227638

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Publication

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_AchLightR

Plasmid

#187463

Purpose

Expresses red acetylcholine indicator AchLightR in neuronal cells

Depositor

Tommaso Patriarchi

Publication

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AchLightR

Use

AAV

Tags

Igκ leader

Promoter

human Synapsin-1

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pEY17

Plasmid

#191023

Purpose

flp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-18 CELE_Y48D7A.2 (flp-18 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-iGECInano

Plasmid

#186189

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Bacterial

Promoter

AraC

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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3TERM_35S

Plasmid

#136114

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFTSG

Plasmid

#162389

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

His

Expression

Insect

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TwinStrep-GFP11

Plasmid

#153514

Purpose

TwinStrep-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TwinStrep-GFP11

Use

Synthetic Biology

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_His2-GFP11

Plasmid

#153511

Purpose

His2-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

His2-GFP11

Use

Synthetic Biology

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Tags

c-Myc and poly-His

Expression

Bacterial

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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T7-AglC-His6

Plasmid

#89726

Purpose

Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Publication

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglC

Tags

His6 and T7

Expression

Bacterial

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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T7-PseB-His6

Plasmid

#89723

Purpose

Expresses PseB from C. jejuni with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Publication

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseB

Tags

His6

Expression

Bacterial

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1 Trx-Bx

Plasmid

#106290

Purpose

Expresses Trx-Bx in Ecoli off a T7 promoter

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Trx-BX

Expression

Bacterial

Promoter

T7

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pJA14

Plasmid

#59929

Purpose

gRNA for cleavage at rde-1(H974) locus in C elegans

Depositor

Andrew Fire

Publication

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(H974) gRNA

Use

CRISPR

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pGR-L3S2P00

Plasmid

#46005

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

L3S2P00 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pHZ149

Plasmid

#27509

Depositor

Andrew Fire

Publication

Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

Insert

pREAR::DsRed::unc-54_3'UTR

Expression

Worm

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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XE157 GFP-XDpr-delta PDZB-CS2+

Plasmid

#16765

Depositor

Randall Moon

Publication

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

XDpr-delta PDZB (dact1-b Frog)

Use

Xenopus expression

Tags

gfp

Mutation

XDpr is truncated to delete the last four amino a…

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-CaBLAM_294W

Plasmid

#244130

Purpose

Lower affinity variant of CaBLAM bioluminescent calcium indicator

Depositor

Nathan Shaner

Publication

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Insert

CaBLAM_294W

Expression

Mammalian

Mutation

Calmodulin N98W

Available since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pBIG2AB-eIF3klefhdm

Plasmid

#240041

Purpose

Human eIF3k, eIF3l, eIF3e, eIF3f, eIF3h, eIF3d and eIF3m expression in insect cells for purification

Depositor

Venki Ramakrishnan

Publication

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3k, l, e, f, h, d, m

Tags

eIF3d: HA

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pmU1-CasRx-non-targeting (pKW83)

Plasmid

#229051

Purpose

Cloning backbone for CasRx crRNA (Mouse U1 promoter)

Depositor

S Chul Kwon

Publication

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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THINGL pcDNA3.1

Plasmid

#230046

Purpose

Expressing the human version of TINGL biosensor for glucose (named THINGL) in mammalian cells

Depositor

Bas Teusink

Publication

Botman et al ACS Sens. 2026 Feb 2. doi: 10.1021/acsse

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

THINGL

Expression

Mammalian

Available since

Jan. 23, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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