Addgene

We narrowed to 4,235 results for: gem

Showing: 511 - 520 of 4235 results

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pGEMHE-mEGFP-Map4

Plasmid

#105571

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

MAP4 (Map4 Mouse)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDNR-SpCas9-Gem

Plasmid

#80424

Purpose

Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Insert

SpCas9-Gem

Use

CRISPR

Tags

Geminin

Expression

Mutation

codon optimized for human

Promoter

T7

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21ΔPRYSPRY

Plasmid

#105524

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Expression

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-AtpU6-26

Plasmid

#160218

Purpose

AtpU6-26 Golden Gate level 0 piece to express gRNAs

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtU6-26 promoter

Use

Golden gate level 0 piece to express grnas

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLBS.EF/miRFP670-hGem110Nt

Plasmid

#129333

Purpose

Cell cycle reporter, lentivirus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

miRFP670 (Addgene 80006)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer2:sHyper2

Plasmid

#84737

Purpose

Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Use

Tags

NLS-SV40

Expression

Plant

Mutation

Promoter

pFMV/p35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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p4020 pGEM HPV-18 E6

Plasmid

#10856

Depositor

Peter Howley

Article

Werness et al Science. 1990 Apr 6. 248(4951):76-9.

Insert

HPV18 E6 (E6 Human papillomavirus)

Use

Tags

HA?

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or9a

Plasmid

#59637

Purpose

Probe for Drosophila melanogaster Or9a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or9a (Or9a Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
p2207 pGEM p53

Plasmid

#10853

Depositor

Peter Howley

Article

Werness et al Science. 1990 Apr 6. 248(4951):76-9.

Insert

p53 (TP53 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pGEM mouse dbx-1 [TJ#11]

Plasmid

#16269

Depositor

Thomas Jessell

Article

Pierani et al Cell. 1999 Jun 25. 97(7):903-15.

Insert

dbx1 3'UTR (Dbx1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only