We narrowed to 2,202 results for: D10

1. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

   Plasmid

   #66708

   Purpose

   expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

   Depositor

   Charles Gersbach

   Insert

   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Expression

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSET-dCas9-actII-4-NT-S1

   Plasmid

   #110185

   Purpose

   Repression of gene expression in Streptomyces by CRISPRi

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Inserts

   dCas9
   sgRNA
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   J23119 and ermE*p

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWJ68

   Plasmid

   #46571

   Purpose

   Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   w-dcas9
   CRISPR array
   

   Use

   CRISPR

   Tags

   rpoZ

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMH220

   Plasmid

   #122836

   Purpose

   dCas9 with GreenGate B-C flanking sequences

   Depositor

   Markus Schmid

   Article

   Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

   Insert

   Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   Contains D10A and H840A mutations

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Yeast_Target-AID3S

   Plasmid

   #188646

   Purpose

   Expresses Target-AID3S in yeasts

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID2S-UGI
   

   Use

   Tags

   UGI

   Expression

   Yeast

   Mutation

   D10A for SpCas9

   Promoter

   pGal1

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EC2_7_dCas9_HC_sgRNA

   Plasmid

   #163711

   Purpose

   High copy plasmid with dCas9 and sgRNA expression cassettes

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL690

   Plasmid

   #47753

   Purpose

   Expresses dCas9-VP64 fusion

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   CMV

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mammalian_SaAID

   Plasmid

   #188653

   Purpose

   Expresses SaAID in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nSaCas9-AID-UGI
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SaCas9

   Promoter

   pCMV

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF-BE4Gam

   Plasmid

   #138269

   Purpose

   Expression of BE4am-Cas9 Base-editor from an EF1a promoter.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

   Insert

   BE4Gam
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Ef1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-myc-MmUbc7/2776

    Plasmid

    #11488

    Depositor

    Allan Weissman

    Article

    Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

    Insert

    MmUbc7 (Ube2g2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pEF:iCas9

    Plasmid

    #138267

    Purpose

    Expression of mTN3-GGS6-dCas9 (iCas9) in human.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mTN3-GGS6-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cas9-D10A, H840A

    Promoter

    Ef1a

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mammalian_SaAID3S

    Plasmid

    #188651

    Purpose

    Expresses SaAID3S and gRNA in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    nSaCas9-AID3S--UGI
    Sa-sgRNA
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    EF-1a and H1

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mammalian_SaAID2S

    Plasmid

    #188652

    Purpose

    Expresses SaAID2S and gRNA in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    AID2S-nSaCas9--UGI
    Sa-sgRNA
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    EF-1a and H1

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. RhoG

    Plasmid

    #55694

    Purpose

    expression of mouse RhoG

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RhoG (Rhog Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pE2F3.3.1-gDNA

    Plasmid

    #113776

    Purpose

    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor E2F3

    Depositor

    Kevin White

    Insert

    E2F3 (E2F3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-myc-MmUbc7mut/2777

    Plasmid

    #11489

    Depositor

    Allan Weissman

    Article

    Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

    Insert

    MmUbc7 (Ube2g2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    cys 889 mutated to ser

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-KG-MmUbc7/2783

    Plasmid

    #11490

    Depositor

    Allan Weissman

    Article

    Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

    Insert

    MmUbc7 (Ube2g2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. peSpdCas9-J23109

    Plasmid

    #113153

    Purpose

    Plasmid constitutively expressing eSpdCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    eSpdCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A & K810A & H840A & K1003A & R…

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFOSGE_COF2G08

    Plasmid

    #70915

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Parg (Parg Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

20. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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