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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,112 results for: cas12a

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Showing: 1021 - 1040 of 1112 results

  1. pCSN071

    Plasmid
    #166729
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Publication
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166729-plasmid-map-sequence-id-332207

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  2. pJK520

    Plasmid
    #155386
    Purpose
    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Availability
    Academic Institutions and Nonprofits only
    155386-plasmid-map-sequence-id-309003

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  3. pCSN072

    Plasmid
    #166730
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Publication
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166730-plasmid-map-sequence-id-332216

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  5. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    155388-plasmid-map-sequence-id-324484

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  11. pYPQAT45

    Plasmid
    #223417
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for dicot plants; TTTV PAM; LbCas12a-D156R and the crRNA was driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-D156R-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223417-plasmid-map-sequence-id-453515

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  12. pYPQAT46

    Plasmid
    #223418
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223418-plasmid-map-sequence-id-454166

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  13. pYPQAT47

    Plasmid
    #223419
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223419-plasmid-map-sequence-id-454168

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  19. pLCHKOv3-mCherry-intergenic-As

    Plasmid
    #209037
    Purpose
    Lentiviral vector expressing mCherry along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209037-plasmid-map-sequence-id-413702

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  20. pMini-CAAGs::RFP-DR48

    Plasmid
    #132640
    Purpose
    Fluorescent reporter to quantify elicitation of the strand annealing mediated repair pathway
    Depositor
    Stephen Ekker
    Publication
    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRFP
    Expression
    Mammalian
    Mutation
    Contains a 92bp insertion interrupting the open …
    Promoter
    Chicken B-actin
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    132640-plasmid-map-sequence-id-260403

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Showing: 1021 - 1040 of 1112 results