We narrowed to 2,803 results for: cdna expression plasmid

1. pcDNA3-Lyn-dInPAkt(R23/25A)

   Plasmid

   #181835

   Purpose

   Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-dInPAkt
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase, dd…

   Expression

   Mammalian

   Mutation

   Arginines 23 and 25 in PH domain mutated to alani…

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

   Plasmid

   #114111

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

   Insert

   Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.2 mir-1-1 reporter dme-mir-2a-1

   Plasmid

   #46664

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   dme-mir-2a-1
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 Cas9-P2A-dTomato-PS9

   Plasmid

   #231911

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 Cas9-P2A-dTomato-T20

   Plasmid

   #231912

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.2 mir-1-1 reporter dme-let-7

   Plasmid

   #46662

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   dme-let-7
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.2 mir-1-1 reporter dme-mir-34

   Plasmid

   #46665

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   dme-mir-34
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

   Plasmid

   #216292

   Purpose

   Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras heavy chain-GSG-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT

   Plasmid

   #204694

   Purpose

   This plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   direct fusion of BRD4 and NUT

   Promoter

   CMV

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300

    Plasmid

    #204697

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300 (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    direct fusion of BRD4 to p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)

    Plasmid

    #204700

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300(I) (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    deletion of all TF-bidning domains and HAT in p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)

    Plasmid

    #204699

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300 (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    deletion of all TF-bidning domains in p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(H*IT)

    Plasmid

    #204698

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300(H*IT) fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300(H*IT) (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    D1399Y substitution in p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2 mir-1-1 reporter mir16-1.2

    Plasmid

    #46681

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.2 (MIR16-1 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.2 mir-1-1 reporter mir16-1.3

    Plasmid

    #46682

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.3 (MIR16-1 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.2 mir-1-1 reporter mir16-1.1

    Plasmid

    #46680

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.1 (MIR16-1 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT/TO-APT2-LaG16-3W

    Plasmid

    #234425

    Purpose

    Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT/TO-APT1-LaG16-3W

    Plasmid

    #234424

    Purpose

    Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Lyn-PlcR(K30/32L)

    Plasmid

    #181831

    Purpose

    Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-PlcR (K30L,K32L)
    

    Use

    Tags

    ECFP, EYFP, and N-terminal targeting sequence fro…

    Expression

    Mammalian

    Mutation

    Lysines 30 and 32 in PH domain mutated to leucine.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Lyn-dPlcR(K30/32L)

    Plasmid

    #181833

    Purpose

    Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dPlcR (K30L,K32L)
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Lysines 30 and 32 in PH domain mutated to leucine.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge