We narrowed to 1,247 results for: ncl
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Plasmid#136454PurposeMammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tagDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
TagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trCD4o
Plasmid#203751PurposeEncodes the transmembrane domain from CD4, including its 2 palmitoylation sites, with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.DepositorAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-iRFP670 L1-R5
Plasmid#84390PurposeMultisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsiRFP670ExpressionMammalianPromoterCMV immediate earlyAvailable SinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
PET28-FnCas12a-EP16
Plasmid#183636PurposePET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.DepositorInsertFnCas12a-EP16
UseCRISPRTags6×His TagExpressionBacterialMutationN607R, K613V, N617R, K180S, K660R, D616NAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AtTAS1c-D2-B/c
Plasmid#137883PurposeGateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]DepositorInsertAtTAS1c-D2-B/c
UseGateway-compatible entry cloneMutationA. thaliana TAS1c precursor sequence including a …Available SinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-ER-E2F2-dRb
Plasmid#62805Purposeexpresses an inducible E2F2 mutant that lacks the Rb binding domainDepositorInsertE2F2 (E2F2 Human)
UseRetroviralTagsHA-ErExpressionMammalianMutationdeletion of amino acids 302-437, including the Rb…Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2Flag CMV2-ZO-2-N
Plasmid#27418DepositorInsertZonula Occludens 2 (TJP2 Human)
Tags2xFlagExpressionMammalianMutationDeletion of the carboxy-terminus of ZO-2, so only…Available SinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
gfp-Arkadia/ RNF111 deltaRING
Plasmid#112229PurposeExpresses GFP-tagged enzymatically inactive (delta RING) Arkadia in mammalian cellsDepositorInsert(Arkadia)Rnf111 (Rnf111 Mouse)
TagsGFPExpressionMammalianMutationdelta RING domain deletion (Deletion of the last …Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
His10-B1 Integrin
Plasmid#186139PurposeFor expression of recombinant his10-beta1 integrin cytosolic domain in bacteriaDepositorAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP AH2 tensin
Plasmid#50514PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertTensin (TNS1 Chicken)
TagsGFPExpressionMammalianMutationAA659--761, including actin homology 2 domain of …PromoterCMVAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTol1-U6abc_amhc(myh6)_cmlc2-nmKate
Plasmid#238375PurposeDrives expression of 3 different gRNAs targeting amhc (myh6), and expression of nclear mKate in cardiomyocytes.DepositorInsertnuclear mKate/3 gRNAs targeting amhc
Promotercmlc2 (nmKate); U6 (gRNAs)Available SinceNov. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
cBEST2
Plasmid#234658PurposeExpresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertionDepositorInsertsspCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterkasO*17tss and kasOp*Available SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
WT FLAG-HaloTag-RTEL1_DonorVector
Plasmid#247170PurposeHR Donor for WT C-terminal FLAG-HaloTag-RTEL1 fusion in human cells. Includes Puro selection marker.DepositorInsertWT FLAG-HaloTag-RTEL1 (RTEL1 Human)
PromoterEndogenous RTEL1 promoterAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
K48R FLAG-HaloTag-RTEL1_DonorVector
Plasmid#247171PurposeHR Donor for K48R C-terminal FLAG-HaloTag-RTEL1 fusion in human cells. Includes Puro selection marker.DepositorInsertK48R FLAG-HaloTag-RTEL1 (RTEL1 Human)
PromoterEndogenous RTEL1 promoterAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CR-QUAS:mNeonGreen-NES-P2A-mKate2-NLS
Plasmid#241912PurposeZebrafish transgenesis plasmid encoding cytoplasmic mNeonGreen-NES and mKate2-NLS separated by the P2A peptide, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis markerDepositorInsertQUAS enhancer driving mNeonGreen-NES and mKate2-NLS
UseZebrafish tol2 transgenesisPromoterQUASAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-QUAS:cPla2-mKate2
Plasmid#241913PurposeZebrafish transgenesis plasmid encoding D. rerio cPla2-mKate2, driven by the QUAS enhancer, and including a cmlc2:EGFP cardiac transgenesis markerDepositorInsertQUAS enhancer driving cPla2-mKate2
UseZebrafish tol2 transgenesisTagscPla2 fused to mKate2PromoterQUASAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-mTLR4ecd-VLR
Plasmid#187877PurposeBaculovirus protein expression of mouse TLR4 (residues 26–544) fused with hagfish variable lymphocyte receptor (VLR) (residues 126–200)DepositorAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
enDR3-TEV-mEGFP-NLS (pRS1451)
Plasmid#240546PurposeExpression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.DepositorInsertenDR3
ExpressionMammalianMutationL52MAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l2-A'-IRES-EmGFP
Plasmid#209125PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFPDepositorInsertSUMO-A'-l2-A'-IRES-EmGFP
UseLentiviralExpressionMammalianAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana MAMI
Plasmid#209186PurposepENTR4 plasmid with CDS of Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI, AT5G54110.1) including stop codon for N-terminal epitope tagsDepositorAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only