We narrowed to 1,439 results for: puc57

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pHAND1-T2A-dTom-PGK-Neo

Plasmid

#209838

Purpose

Tomato reporter plasmid targeting the human HAND1 gene

Depositor

Matthew Birket

Article

Lynch et al Stem Cell Res. 2024 Mar 4;77:103342. doi

Insert

HAND1-T2A-dTomato-PGK-Neo-HAND1 (HAND1 Human)

Use

Human targeting

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDB2-hCRY2-Halo-CD4-bla

Plasmid

#189986

Purpose

Donor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

HaloTag

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2232

Plasmid

#158401

Purpose

High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple316 (NR2E1 Human)

Use

Unspecified

Promoter

Ple316

Available Since

Feb. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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AA041

Plasmid

#215963

Purpose

Fragmid fragment: (Pol 2 promoter) human core (short) EF1a promoter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

EFS_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA294

Plasmid

#215968

Purpose

Fragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U1a_v1

Use

Fragment

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA332

Plasmid

#216053

Purpose

Fragmid fragment: (C' terminus) 3x NLS sequences

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA179

Plasmid

#215939

Purpose

Fragmid fragment: (guide cassette) short sequence with no known function

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Filler

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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EPB104

Plasmid

#68369

Purpose

contains human U6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

U6 promoter (RNU6-1 Human)

Expression

Bacterial

Promoter

U6

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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AA011

Plasmid

#215973

Purpose

Fragmid fragment: (N' terminus) for C>T base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA010

Plasmid

#216038

Purpose

Fragmid fragment: (C' terminus) for C>T base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

UGI_v1.1; NpNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA013

Plasmid

#215975

Purpose

Fragmid fragment: (N' terminus) for A>G base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA302

Plasmid

#216052

Purpose

Fragmid fragment: (C' terminus) Nucleoplasmin NLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.2; Stop; polyA_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-PAL1C

Plasmid

#196685

Purpose

Rep/Cap plasmid for the production of PAL1C, an AAV capsid with CNS tropism in macaques.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

PTQGTLR insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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AA299

Plasmid

#216029

Purpose

Fragmid fragment: (Cas protein) NNGG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9_v1.1 [Slug]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA296

Plasmid

#216028

Purpose

Fragmid fragment: (Cas protein) NNNNCC PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Nme2Cas9_v1.1 [Nme2]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA301

Plasmid

#216030

Purpose

Fragmid fragment: (Cas protein) NNGG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9_v1.1 [Sauri]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)

Plasmid

#65524

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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AA345

Plasmid

#216031

Purpose

Fragmid fragment: (Cas protein) Cas13d

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas13d_v1.1 [Dj]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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