We narrowed to 45,592 results for: cha;

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pGEX-4T Doc2beta C2AB(125) WT

Plasmid

#134690

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression and purification

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluf

Plasmid

#106121

Purpose

Fast indicator for synaptic glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25D variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Glu 25 to Asp

Promoter

synapsin

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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OVA-miSFIT-2xPerfect

Plasmid

#124673

Purpose

Tuning expression of cytosolic Ovalbumin coupled to EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

Cyto-Ovalbumin

Expression

Mammalian

Mutation

Truncated OVA for cytoplasmic expression

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMT2-MEKK3-K392A

Plasmid

#21585

Depositor

John Kyriakis

Article

Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

Insert

mitogen-activated protein kinase kinase kinase 3 (MAP3K3 Human)

Expression

Mammalian

Mutation

Lysine 392 to Arginine

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
DsRed +9e

Plasmid

#191766

Purpose

Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Tags

N terminal fusion of positively charged polypetod…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP-YPet-RanGAP*

Plasmid

#175245

Purpose

Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETa-mKikGR-V69T

Plasmid

#98574

Purpose

photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mKikGR-V69T

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-222-PGK-GFP-Blast

Plasmid

#31522

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-222 (Mir222 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-shDusp5-2-H2Kb2

Plasmid

#17853

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

shDusp5 (DUSP5 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgITGB1-1

Plasmid

#121533

Purpose

sgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

Insert

sgITGB1-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2_1-395_V

Plasmid

#147808

Purpose

Mammalian Expression of HsDCP2_1-395

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_1-395 (DCP2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GST-PIWI(aa478-860)

Plasmid

#21542

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

GST

Expression

Mammalian

Mutation

contain only aa478-860

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pSLQ1371-sgITGB4-1

Plasmid

#121535

Purpose

sgITGB4-1 sequence: GAGGCGCAGTCCTTATCCACA. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

Insert

sgITGB4-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB222

Plasmid

#68613

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB219

Plasmid

#68610

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(human)(R332P)

Plasmid

#79037

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human R332P) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Arg 332 to Pro

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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SNX3-PX (1-162)

Plasmid

#119084

Purpose

Bacterial expression of human phox homology (PX) domain, SNX3-PX (1-162)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX3-PX (1-162) (SNX3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-497/miR-195

Plasmid

#64236

Purpose

Expresses miR-497 and miR-195 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-497 and miR-195 (MIR195, MIR497 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLJC6-Flag CASTOR1 I280A

Plasmid

#84497

Purpose

Lentiviral expression of CASTOR2 I280A mutant

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 I280A (GATSL3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

I280A

Promoter

sestrin2

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-PAK2 S20A

Plasmid

#31672

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Serine 20 to Alanine

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only