We narrowed to 15,044 results for: invs

1. AdMKP-3 S159A

   Plasmid

   #131520

   Purpose

   Expresses MKP-3 with S159A mutation in mammalian cells

   Depositor

   Haiyan Xu

   Article

   Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

   Insert

   Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
   

   Use

   Adenoviral

   Tags

   No

   Expression

   Mutation

   changed Serine 159 to Alanine

   Promoter

   CMV

   Available Since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA tagged mid ELL2

   Plasmid

   #127274

   Purpose

   Expresses human ELL2 with HA tag in the middle that does not disrupt function

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 with HA at XbaI (ELL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HA inserted at XbaI after Met 186

   Promoter

   pEF1a

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. COOHend ELL2

   Plasmid

   #127255

   Purpose

   Expresses the C terminal segment of human ELL2 in mammalian cells

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (C-terminal end) (ELL2 Human)
   

   Use

   Other

   Tags

   Expression

   Mammalian

   Mutation

   contains only the last 389 aa

   Promoter

   pEF1a

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. NH2end ELL2

   Plasmid

   #127252

   Purpose

   Expresses the N terminal segment of human ELL2 in mammalian cells

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (1-287) (ELL2 Human)
   

   Use

   Other

   Tags

   His6X

   Expression

   Mammalian

   Mutation

   contains only amino acids 1-289

   Promoter

   pEF1a

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR

   Plasmid

   #121836

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

   Plasmid

   #121837

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1

   Plasmid

   #121838

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

   Plasmid

   #121840

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

   Plasmid

   #121803

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + EF1a::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid

    #121807

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + RIP::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

    Plasmid

    #121811

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid

    #121815

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid

    #121820

    Purpose

    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JH204: pMVP (R4-R3) human PDX1 open

    Plasmid

    #121745

    Purpose

    pMVP R4-R3 entry plasmid, contains human PDX1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Human PDX1 (open) (PDX1 Human)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

    Plasmid

    #121748

    Purpose

    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    OLLAS epitope tag-IRES2-eGFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pK_P-GAP-LM_lacO-cP-AOX1-sAR

    Plasmid

    #126745

    Purpose

    yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    slovA,cpr
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available Since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pK_P-ICL1-LM_lacO-cP-AOX1-sAR

    Plasmid

    #126744

    Purpose

    yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    slovA,cpr
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    ICL1

    Available Since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCRII apoBb.1

    Plasmid

    #121898

    Purpose

    Plasmid for making zebrafish apoBb.1 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoBb.1 (apobb.1 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCRII apoA-IVb.1

    Plasmid

    #121903

    Purpose

    Plasmid for making zebrafish apoA-IVb.1 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoa4b.1 (apoa4b.1 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PCRII apoA-IVb.2

    Plasmid

    #121904

    Purpose

    Plasmid for making zebrafish apoA-IVb.2 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoa4b.2 (apoa4b.2 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge