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We narrowed to 21,402 results for: flag

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Showing: 10461 - 10480 of 21402 results

  2. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70707-plasmid-map-sequence-id-127770

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  6. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid
    #98041
    Purpose
    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sa-dCas9-NLS-3xFLAG
    Use
    CRISPR
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    D10A + H557A
    Promoter
    CMV
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98041-plasmid-map-sequence-id-398173

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  7. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82613-plasmid-map-sequence-id-298740

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  8. nesprin TSmod 25

    Plasmid
    #125555
    Purpose
    Nesprin-2G force sensor with cerulean-F25-venus TSmodule
    Depositor
    Daniel Conway
    Publication
    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mini-nesprin-2G (Syne2 Mouse)
    Tags
    cerulean, venus
    Expression
    Mammalian
    Mutation
    TSmod changed from mTFP to cerulean, flageliform …
    Promoter
    CMV
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125555-plasmid-map-sequence-id-244038

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  10. pFLAG-CMV2-Hsp27-S78D/S82D

    Plasmid
    #85187
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S78D/S82D, phosphomimicking mutation
    Promoter
    CMV
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    85187-plasmid-map-sequence-id-173286

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  13. CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid
    #171381
    Purpose
    Expression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized Cas13Y.1
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171381-plasmid-map-sequence-id-337347

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  15. pYPQ151

    Plasmid
    #69302
    Purpose
    Gateway entry vector with pcoCas9D10A
    Depositor
    Yiping Qi
    Publication
    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pcoCas9D10A (Plant codon-optimized)
    Use
    CRISPR
    Tags
    2XFLAG
    Mutation
    D10A mutation; NLS fusion
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69302-plasmid-map-sequence-id-122140

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Showing: 10461 - 10480 of 21402 results