We narrowed to 10,569 results for: fus
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Plasmid#176236PurposePlasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-5Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176237PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176238PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
Tags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::T48-Baz::mCherry
Plasmid#163927PurposeExpression of GrabFP-A-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24-ORF24-2xStrep
Plasmid#138453PurposeExpresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24-ORF24 (ORF24 MHV68/KSHV)
TagsStrepExpressionMammalianMutationmu24 amino acids 1-191 fused to ORF24 amino acids…PromoterCMVAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-BcRF1-ORF24-2xStrep
Plasmid#138454PurposeExpresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cellsDepositorTagsStrepExpressionMammalianMutationBcRF1 amino acids 1-178 fused to ORF24 amino acid…PromoterCMVAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-UL87-ORF24-2xStrep
Plasmid#138455PurposeExpresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cellsDepositorTagsStrepExpressionMammalianMutationUL87 amino acids 1-248 fused to ORF24 amino acids…PromoterCMVAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-UD-mCherr
Plasmid#140307PurposeExpresses Flag-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X HAExpressionMammalianMutationcontains only the SH4 and UD regions fused to mCh…PromoterCMVAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-TOM20MTS-DDRGK1-dTM-HA
Plasmid#139861PurposeLentiviral expression of TOM20MTS-DDRGK1-dTM-HADepositorInsertTOM20-MTS-DDRGK1-dTM (DDRGK1 Human)
UseLentiviralTagsHAMutationrecombinant fusion of mitochondria-targeting sign…Available SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV-STAT31F
Plasmid#134725PurposeExpresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707DepositorTagsFLAG and human STAT3 aa 1-514 joined to human STA…ExpressionMammalianAvailable SinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV-STAT13F
Plasmid#134723PurposeExpresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope tag at the C-terminus. Based on addgene plasmids #8691 and #8707DepositorTagsFLAG and human STAT1 aa1-508 joined to human STAT…ExpressionMammalianAvailable SinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-CSNK1A1 G40N-P2A-Blast
Plasmid#123321PurposeLentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoterDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationchanged Glycine 40 to AsparaginePromoterCMVAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (L313D I314K)
Plasmid#121409PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationLeucine 313 to Aspartic acid; Isoleucine 314 to …PromoterCUP1 promoterAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-2xTS
Plasmid#117750PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b+C3(R112C/T180C)X
Plasmid#108196PurposeExpresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coliDepositorInsertP450cam-PCNA3(R112C/T180C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPD3cC
Plasmid#86972PurposeExpresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3R112C/T180CHFm
Plasmid#85091PurposeExpresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
TagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3, A74G in heme-FMN domain of …PromoterT7 promoterAvailable SinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha
Plasmid#33330DepositorInsertCalcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
TagsFLAGExpressionMammalianMutationCaMKKbeta N-terminus (1-164) was fused to body fo…PromoterSV40Available SinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -