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pIE-C-hα2M-H6

Plasmid

#128494

Purpose

Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (R787-N1473) (A2M Human)

Tags

His tag (6x)

Expression

Insect

Mutation

contains R787-N1473

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Ataxin-1, 11NQ [N76/3R]

Plasmid

#114559

Purpose

Mammalian Expression Plasmid of anti-Ataxin-1, 11NQ (Mouse). Derived from hybridoma N76/3.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Ataxin-1, 11NQ (Mus musculus) recombinant mouse monoclonal antibody (Atxn1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-TRAF4(K190R)

Plasmid

#58317

Purpose

FLAG-tagged mouse TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pClneoMyc MOB1 T12A

Plasmid

#47936

Purpose

expresses Myc tagged human MOB1 containing T12A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Tags

Myc

Expression

Mammalian

Mutation

T12A

Promoter

CMV

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
AAV-eGFP-KASH

Plasmid

#248781

Purpose

AAV construct expressing eGFP-KASH enabling FACS-sorting of extracted nuclei

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

eGFP-KASH

Promoter

Cbh

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST14-DoubleCatcher-H-Lock

Plasmid

#216285

Purpose

Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

DoubleCatcher H-Lock

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-9-35S-11

Plasmid

#197759

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET303-colE1T

Plasmid

#155180

Purpose

Expresses colicin E1 residues 1-190

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-190

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-190

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET303-colE1TR

Plasmid

#155181

Purpose

Expresses colicin E1 residues 1-364

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-364

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-364

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8_MF-p10-mCh (VE5626)

Plasmid

#139772

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with mCherry cDNA under p10 to monitor infection.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

mCherry fluorescent protein

Promoter

PH

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLXMA_RK2 L2A

Plasmid

#153361

Purpose

Expression vector for plants with low-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTKA001

Plasmid

#164577

Purpose

Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

Depositor

Robert Kirchdoerfer

Article

Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

Insert

nsp7 (ORF1ab )

Tags

6xHis, Ek, TEV

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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U6b-T

Plasmid

#117210

Purpose

Expresses gRNA in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

Insert

u6b promoter-gRNA

Use

Synthetic Biology

Expression

Insect

Promoter

U6

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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U6c-T

Plasmid

#117211

Purpose

Expresses gRNA in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

Insert

u6c promoter-gRNA

Use

Synthetic Biology

Expression

Insect

Promoter

U6

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDOE11 Lat52:mCherry transient

Plasmid

#124249

Purpose

Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.

Depositor

Attila Fehér

Article

Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

Type

Empty backbone

Expression

Plant

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEW65: pQE-81L H3(K79M)

Plasmid

#124100

Purpose

H3K79M expression plasmid for norleucine supplementation

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Histone H3 (LOC108704296 Frog)

Expression

Bacterial

Mutation

K79M

Promoter

T5

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304 LacZ-V5 blast

Plasmid

#83099

Purpose

Lentiviral vector for constitutive expression of LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pMM1157

Plasmid

#112534

Purpose

pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
luxCDABE

Expression

Bacterial

Promoter

PL(HrtO) and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2048

Plasmid

#79656

Purpose

ssAAV genome with Ple259 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple259-EmGFP WPRE

Use

AAV

Promoter

PAX6 MiniPromoter

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2045

Plasmid

#79653

Purpose

ssAAV genome with Ple256 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple256-EmGFP WPRE

Use

AAV

Promoter

PAX6 enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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