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T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

Plasmid

#193356

Purpose

VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F

Plasmid

#200877

Purpose

ZooTsy_TnsF expression construct for protein purification.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy_TnsF

Expression

Bacterial

Mutation

TnsF-Y584F

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF

Plasmid

#200865

Purpose

AjTn6022_TniQ expression construct for protein purification.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022_TnsF

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAM_VHS

Plasmid

#109834

Purpose

Protein expression and purification of STAM_VHS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

STAM_VHS (STAM Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPRED1_WH1

Plasmid

#109816

Purpose

Protein expression and purification of SPRED1_WH1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPRED1_WH1 (SPRED1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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C-term png in pGEX4

Plasmid

#112975

Purpose

For protein expression and purification of the C-term of Drosophila png

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png (png Fly)

Expression

Bacterial

Mutation

wild-type C terminus

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTSin EGFP

Plasmid

#127695

Purpose

Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

EGFP

Use

Synthetic Biology

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Str-KDEL_ST-SBP-EGFP

Plasmid

#65264

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCDF-1b-RFC140ΔN555

Plasmid

#175043

Purpose

Overexpression of human (Rfc1) p140ΔN555 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 1 (RFC1 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-mCherry

Plasmid

#65265

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pWZL Blast DN E-cadherin

Plasmid

#18800

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

Dom neg E-cadherin (Cdh1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

truncated form of E-cadherin (dominant negative) …

Available Since

July 15, 2008

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-EGFP

Plasmid

#65258

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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PTP1B human

Plasmid

#102719

Purpose

human PTP1B catalytic domain (residues 1-301) in RP1B vector

Depositor

Wolfgang Peti

Article

Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5.

Insert

Protein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-mCherry

Plasmid

#65259

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(C163S)

Plasmid

#90088

Purpose

Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

C163S

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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BPK1179

Plasmid

#179296

Purpose

Expresses dCas9 fused to four DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-DmrA(x4)

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ST-SBP-mCherry

Plasmid

#65269

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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ST-SBP-mCherry_puromycin

Plasmid

#65271

Purpose

ST reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pSH1/Mf-del-Akt-E

Plasmid

#15288

Depositor

David Spencer

Article

Park et al Nat Biotechnol. 2006 Dec . 24(12):1581-9

Insert

delPH-Akt (Akt1 Mouse)

Tags

HA epitope and Myristoylation-targeting domain Fy…

Expression

Mammalian

Mutation

Truncated PH (residues 1-99)

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-tagBFP

Plasmid

#65266

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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