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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,044 results for: invs

Showing: 10561 - 10580 of 15044 results

  2. pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

    Plasmid
    #138299
    Purpose
    Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKCsub(T/A)-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Availability
    Academic Institutions and Nonprofits only
    138299-plasmid-map-sequence-id-307463

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  4. Dronpa-FHA1

    Plasmid
    #87711
    Purpose
    Encodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Dronpa-FHA1
    Use
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87711-plasmid-map-sequence-id-176141

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  6. CMV-mito-R-GECO1

    Plasmid
    #46021
    Purpose
    Red intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    R-GECO1
    Use
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    46021-plasmid-map-sequence-id-350822

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  8. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid
    #227321
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227321-plasmid-map-sequence-id-452094

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  10. FH-Tet2-pEF

    Plasmid
    #41710
    Purpose
    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system
    Depositor
    Anjana Rao
    Article
    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi
    Insert
    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    Use
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    41710-plasmid-map-sequence-id-322448

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  17. pDisplay-EGFP-CAAX

    Plasmid
    #208683
    Purpose
    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208683-plasmid-map-sequence-id-412176

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  18. pDisplay-GRAB_CRFmut

    Plasmid
    #208656
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    208656-plasmid-map-sequence-id-410341

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Showing: 10561 - 10580 of 15044 results