We narrowed to 22,515 results for: tes

1. pAAV-S5E2-C1V1-eYFP (AAV PHP.eB)

   Viral Prep

   #135633-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-C1V1-eYFP (#135633). In addition to the viral particles, you will also receive purified pAAV-S5E2-C1V1-eYFP plasmid DNA. Expression of red-shifted opsin C1V1 fused to EYFP under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Tags

   EYFP

   Available Since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

2. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Expression

   Mammalian

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-CMV-Luc2-IRES-ZsGreen-W

   Plasmid

   #164432

   Purpose

   Lentiviral vector backbone encoding firefly luciferase and ZsGreen

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Inserts

   Luciferase
   ZsGreen
   

   Use

   Lentiviral

   Available Since

   Feb. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTet-O-ASCL1-T2A-PuroR

   Plasmid

   #162345

   Purpose

   Lentiviral expression of ASCL1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   ASCL1 (ASCL1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Promoter

   Tet-ON

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mKate2-DrCavin1b in pT3TS-Dest

   Plasmid

   #194295

   Purpose

   In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   cavin1b (cavin1b Zebrafish)
   

   Use

   In vitro transcription of mrna

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHDM-Hgpm2

   Plasmid

   #164441

   Purpose

   Encodes codon-optimized HIV gagpol for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   Codon Optimized HIV-1 GagPol
   

   Expression

   Mammalian

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-EEA1

   Plasmid

   #109560

   Purpose

   Multisite gateway vector for 5' tagging with early endosome associated 1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   early endosome associated 1 (eea1 Zebrafish)
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRC-CMV-Rev1b

   Plasmid

   #164443

   Purpose

   Encodes HIV-1 Rev accessory protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   HIV-1 Rev
   

   Expression

   Mammalian

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHDM-VSV-G

   Plasmid

   #164440

   Purpose

   Encodes VSV-G protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   VSV-G
   

   Expression

   Mammalian

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHDM-Tat1b

    Plasmid

    #164442

    Purpose

    Encodes HIV-1 Tat accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Tat
    

    Expression

    Mammalian

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-ExRai-AKAR2

    Plasmid

    #161753

    Purpose

    Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

    Plasmid

    #132771

    Purpose

    Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

    Depositor

    Jan Lammerding

    Article

    Denais et al Science. 2016 Apr 15;352(6283):353-8. do

    Insert

    Cyclic GMP-AMP synthase (CGAS Human)
    

    Use

    Lentiviral

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    E225A D227A

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p5E-elavl3

    Plasmid

    #75025

    Purpose

    Can be used to drive pan neuronal expression in zebrafish.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Promoter

    elavl3

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag‐mTim3

    Plasmid

    #49208

    Purpose

    expresses Flag tagged mouse Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (Havcr2 Mouse)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    no Tim3 signal sequence

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. huCaV2.2 (pSAD442-1)

    Plasmid

    #62574

    Purpose

    Human CaV2.2 expression in mammalian cells

    Depositor

    Diane Lipscombe

    Article

    Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

    Insert

    CACNA1B (CACNA1B Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

    Plasmid

    #61590

    Purpose

    Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

    Depositor

    David Komander

    Article

    Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

    Insert

    TRABID (ZRANB1 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-244 and aa 698-708.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINK-OTUD3 (OTU, aa 52-209)

    Plasmid

    #61412

    Purpose

    Expresses human OTUD3 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 210-398.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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