We narrowed to 109,790 results for: MAL

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FLAG-hIes6

Plasmid

#15356

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

hIes6 (INO80C Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pET303-ColE1-T-E192C

Plasmid

#180233

Purpose

Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T domains (residue…

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–dSpCas9

Plasmid

#157833

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-205

Plasmid

#26312

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-205 (MIR205 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pJM600 EF1α PC 3xFLAG-FKBP-FGFR4-TEVp in TUPV2

Plasmid

#161570

Purpose

Constitutive expression of PC 3xFLAG-FKBP-FGFR4-TEVp under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

PC 3xFLAG-FKBP-FGFR4-TEVp

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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CLPIT Cry2PHR-mCh-RhoA

Plasmid

#42959

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)

Use

Retroviral

Expression

Mammalian

Promoter

Tet-Off promoter

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSyn1-EGFP

Plasmid

#153187

Purpose

Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-CAG-EGFP

Plasmid

#153186

Purpose

CAG promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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nTRb412-gs10-NLS-VPR

Plasmid

#188267

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split TRbeta (aa 200-412), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nTRb412 (TXNRD2 Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

Plasmid

#168216

Purpose

Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

SV40 NLS SpCas9

Use

Lentiviral

Tags

HA, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E W73A IRES GFP

Plasmid

#18762

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E W73A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

W73A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-dCpf1-eBE-YE

Plasmid

#107689

Purpose

Expresses dLbCpf1-eBE-YE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-eBE-YE

Expression

Mammalian

Mutation

APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

Promoter

CMV

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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ABI_cPPVp

Plasmid

#119212

Purpose

Expresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cPPVp in fusion with ABI

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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PYL1_nPPVp

Plasmid

#119211

Purpose

Expresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nPPVp in fusion with PYL1

Tags

AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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FRB_nSbMVp

Plasmid

#118969

Purpose

Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nSbMVp in fusion with FRB

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_051

Plasmid

#123948

Purpose

Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hAAVS1 landing pad destination vector

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP718nano

Plasmid

#195750

Purpose

Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

H2B-miRFP718nano

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

Plasmid

#167898

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

Plasmid

#170182

Purpose

Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

Depositor

Hunter Fraser

Article

Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

Insert

mCherry

Tags

3XFlag and T2A

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-GW182delta1

Plasmid

#11592

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

GW182 (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contains only aa1-498; SEE DEPOSITOR NOTES BELOW

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only