We narrowed to 28,641 results for: Tat

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pEF6-Lck-TurboID

Plasmid

#159433

Purpose

expresses Lck-TurboID in mammalian cells

Depositor

Arthur Salomon

Article

Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

Inserts

Lck (LCK Human)
TurboID (BirA mutant)

Tags

HA

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

EF-1a

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-10-3xFLAG-LGP2-K651E

Plasmid

#58685

Purpose

Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells

Depositor

Curt Horvath

Article

Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

Insert

LGP2-K651E (DHX58 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

mutation to CTD, K651E

Promoter

CMV

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only
pMCSF-R(mB)-luc

Plasmid

#12422

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Mutation

AML1 binding site mutation (created a KpnI site i…

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pLX317-MBNL1

Plasmid

#115443

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL1 (MBNL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pC-G418-YR

Plasmid

#61767

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

nptII gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

trpC promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pU6_HEK3_nsgRNA(PP7)

Plasmid

#232444

Purpose

nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

Plasmid

#207805

Purpose

E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

Depositor

Koen Breyne

Article

Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

Insert

E-NoMi

Use

Lentiviral

Tags

3xFlag tag, copGFP, mCherry, and nanoluciferase

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast GFP-SPNS1

Plasmid

#198571

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-PGBD5

Plasmid

#78121

Purpose

Lentiviral gene expression vector for doxycycline inducible PGBD5 expression

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

PGBD5 (PGBD5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPA8

Plasmid

#19541

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 8 (HSPA8 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pLX304 ERG

Plasmid

#118625

Purpose

Overexpresses ERG protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG (33-479) (ERG Human)

Use

Lentiviral

Tags

None and none

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Tau(T217E)

Plasmid

#187024

Purpose

Expresses EGFP tagged human 2N4R tau (with T217E mutation) in mammalian cells with CMV promoter

Depositor

George Bloom

Article

Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Threonine 217 to Glutamic acid

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pInducer20 ALPK1 Y254C

Plasmid

#231587

Purpose

ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

changed tyrosine 254 to cysteine

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1

Plasmid

#160941

Purpose

Generation of retrovirus for the overexpression of Nr4a1

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K11

Plasmid

#121152

Purpose

Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

All Ks apart from K11 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1-C450A

Plasmid

#22028

Purpose

Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL B WT

Plasmid

#202414

Purpose

Expression of GFP-tagged PODXL (isoform B) WT

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx46-IRES-GCaMP6s

Plasmid

#188238

Purpose

3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6s

Depositor

Fabio Mammano

Article

Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

Insert

GJA3 (GJA3 Human)

Use

Lentiviral

Tags

GCaMp6s

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-DEST

Plasmid

#119746

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

Depositor

Andrew Jackson, Martin Reijns

Article

Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pInducer20 ALPK1 V1092A

Plasmid

#231588

Purpose

ALPK1 gene mutated in position V1092A (mutation found in spiradenomacarcinoma (sweat gland cancer)) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

changed Valine1092 to Alanine

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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