We narrowed to 29,279 results for: Tat

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pGEX6P1-NEDD4L_FL-CS

Plasmid

#187713

Purpose

Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

Cys874 mutated to a serine

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCKC017

Plasmid

#226622

Purpose

pLenti wt hTdT template for libraries 1-2

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

Terminal deoxynucleotidyl transferase (DNTT Human)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

synonymous mutation in D2, synonymous mutation in…

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry

Plasmid

#226101

Purpose

HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

HRI (EIF2AK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

SIFI degron helices 1 & 2 deleted (Δ61-112)

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcs2-N-HRI(1-138)-GFP-IRES-mCherry

Plasmid

#226100

Purpose

HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

HRI (EIF2AK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

contains only N-HRI (aa 1-138)

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL FBM*/PBM*

Plasmid

#195221

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Expression

Mammalian

Mutation

Dual mutant for HRS>AAA / lacking DTHL

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 WT

Plasmid

#185632

Purpose

expression of GST-tagged wild-type PML B-box1 domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 C4C4

Plasmid

#185640

Purpose

expression of GST-tagged mutant PML B-box1 H155C/H161C domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

H155C H161C

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 W157E

Plasmid

#185641

Purpose

expression of GST-tagged mutant PML B-box1 W157E domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

W157E

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 F158E

Plasmid

#185642

Purpose

expression of GST-tagged mutant PML B-box1 F158E domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

F158E

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKS732A/S910A

Plasmid

#221642

Purpose

This retroviral plasmid expresses a double-mutated cDNA (dominant negative: S732A/S910A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S732A/S910A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK double mutation S732A/S910A (dominant negativ…

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKP712/713A

Plasmid

#216544

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: P712/713A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (P712/713A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation P712/713A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKR177/178A

Plasmid

#216543

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: R177/178A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (R177/178A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation R177/178A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-LRPPRC-ts3

Plasmid

#174270

Purpose

LRPPRC knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

LRPPRC shRNA (LRPPRC Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX3X-ts1

Plasmid

#174235

Purpose

DDX3X knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX3X shRNA (DDX3X Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pACB120

Plasmid

#222213

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA(K10N, S11R, W15C, G76V, G258D)

Tags

6x His

Expression

Bacterial

Mutation

Contains five amino acid mutations: K10N, S11R, W…

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

Plasmid

#206178

Purpose

Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

Depositor

Yuxuan Guo

Article

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Cre

Use

AAV

Promoter

cTnT

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-MCHpr-DREADD (Gq)-mCherry

Plasmid

#221036

Purpose

Expresses Gq coupled DREADD hM3D under the rat pro-melanin-concentrating hormone promoter

Depositor

Yanhua Huang

Article

Wang et al Mol Psychiatry. 2021 Jul;26(7):3152-3168

Insert

DREADD Gq-mCherry

Use

AAV; Rat targeting

Expression

Mammalian

Promoter

Rat MCH

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-3xFlag-SPNS1

Plasmid

#198568

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag (SPNS1 Human)

Use

Retroviral

Tags

3xFlag

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

Plasmid

#131684

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Promoter

EF1a and mU6-LSL (Cre dependent)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-FAM13A

Plasmid

#185550

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting FAM13A

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

FAM13A gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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