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pMSCVpuro-Gcn5

Plasmid

#63706

Purpose

retroviral expression of mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

None

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pDEST-HemmarR

Plasmid

#31222

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdTom (CD4 Aequorea victoria, Fly, Human)

Tags

CD4 and tdTomato

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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902 pLNCX myr HA Akt1 K179M

Plasmid

#9006

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 K179M (AKT1 Human)

Use

Retroviral

Tags

HA and myr

Expression

Mammalian

Mutation

K179M. kinase dead.

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pCAGGS 3xFLAG Mbnl2

Plasmid

#217032

Purpose

Overexpression of Mbnl2 in mammalian cells

Depositor

Chaolin Zhang

Article

Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

Insert

Mbnl2 (Mbnl2 Mouse)

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4-FLAG-Jhdm2a-H1122A

Plasmid

#38140

Depositor

Toshinobu Nakamura, Toru Nakano

Article

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Insert

Jhdm2a-H1122A (Kdm3a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115; H1122A

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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Nxph4-2A-CreERT2 Targeting Vector

Plasmid

#62219

Purpose

Targets CreERT2 to the mouse Nxph4 stop codon

Depositor

Hongkui Zeng

Article

Harris et al Front Neural Circuits. 2014 Jul 10;8:76.

Insert

Nxph4-2A-CreERT2 (Nxph4 Bacteriophage, Mouse)

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

Mouse Nxph4

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-DAP5-3XFLAG

Plasmid

#127330

Purpose

Express 3xFLAG tagged DAP5 protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-EPCP5

Plasmid

#228879

Purpose

Expression of Z-tagged LRRK2 KW using Baculoviruses

Depositor

Sebastian Mathea

Article

Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

His-Z-TEV

Expression

Insect

Mutation

Amino acids deleted 1-1846

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLex 983-Micu2

Plasmid

#50372

Purpose

Expression in mammalian cells

Depositor

Vamsi Mootha

Article

Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

Insert

MICU2 (MICU2 Human)

Use

Lentivirus

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
FRB-GFP-Gab1(Y628F/Y660F)

Plasmid

#188658

Purpose

FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 1 (Gab1 Mouse)

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y628F and Y660F

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-DDX54-FLAG/HA

Plasmid

#97062

Purpose

Expresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Tags

FLAG/HA

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pT2K-LRL-IRES-Luc2

Plasmid

#109230

Purpose

For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

LRL and Luc2

Expression

Mammalian

Promoter

CAGGS

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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PB-Neo-TetO-Cdk12_Untagged

Plasmid

#127178

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Piggybac transposase vector

Tags

None

Expression

Mammalian

Promoter

TetO Promoter

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo/Cre

Plasmid

#67594

Purpose

Expresses an Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgNeo

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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1017 pcDNA3 Myr Akt1 deltaPH

Plasmid

#9009

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 dPH (AKT1 Human)

Tags

myr (MGSSKSKPKDPSQR)

Expression

Mammalian

Mutation

delta PH domain (aa1-130)

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Aequorea victoria, Fly, Human)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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FLAG-MED19

Plasmid

#15420

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
SYKA-c020

Plasmid

#175489

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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FRB-GFP-Gab2(Y604F/Y634F)

Plasmid

#188659

Purpose

FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 2 (Gab2 Mouse)

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y604F and Y634F

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-STAT3C-3xFlag

Plasmid

#99264

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

A662C N664C

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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