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pWS067

Plasmid

#123044

Purpose

_-factor GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste2 [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS053

Plasmid

#123025

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFIG1 [2]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS060

Plasmid

#123024

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFUS1 [2]

Expression

Yeast

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB4c-GFP

Plasmid

#35350

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProE

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbS4c-RFP

Plasmid

#35302

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProE

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_2

Plasmid

#63803

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_52

Plasmid

#63808

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_37

Plasmid

#63807

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1

Plasmid

#91942

Purpose

Bacterial expression of maize SAE1

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

HA-ZmSAE1

Tags

HA

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGC276

Plasmid

#19637

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pBXNAC3H

Plasmid

#47074

Purpose

FX cloning E.coli expression vector with araBAD promoter N-terminal Avi-tag, C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGC163

Plasmid

#19635

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC225

Plasmid

#19636

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)<4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pBbA3c-RFP

Plasmid

#35308

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProS

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pNH043

Plasmid

#42155

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pBbE4c-RFP

Plasmid

#35296

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProE

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pGC160

Plasmid

#19632

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAB166

Plasmid

#101668

Purpose

Opto-T7RNAP*(563-F2), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB171

Plasmid

#101670

Purpose

Opto-T7RNAP*(69), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PAJ408

Plasmid

#104150

Purpose

dCas9 plasmid expressing full length mKate2 mRNA without RBS

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO-1

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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