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pSL2623 (pCas6_Vch)

Plasmid

#200895

Purpose

Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas6

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) EC Her2-Fc

Plasmid

#183250

Purpose

Soluble expression of the extracellular domain of the HER2 antigen. Human IgG1 Fc.

Depositor

Alexander McLellan

Article

Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

Insert

Her2 antigen Fc

Expression

Mammalian

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGE144 (MutaEco; aTC-induced)

Plasmid

#189824

Purpose

Expresses MutaEco for generating mutations in the E. coli genome (aTC-induced)

Depositor

Seokhee Kim

Article

Eom et al Nucleic Acids Res. 2022 Apr 22;50(7):e38

Insert

EcoRNAPα-PmCDA1

Expression

Bacterial

Promoter

tetR/tetA promoter

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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PLX_307 SLC31A1

Plasmid

#184491

Purpose

Lentivirus expression for SLC31A1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

SLC31A1 (SLC31A1 Human)

Use

Lentiviral

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-BFP-CAAX

Plasmid

#184459

Purpose

Plasma membrane-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_CACNA2D3_OE_1

Plasmid

#161683

Purpose

Overexpresses CACNA2D3

Depositor

Mike McManus

Insert

CACNA2D3 (CACNA2D3 Human)

Tags

V5

Expression

Mammalian

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_TMC5_OE_1

Plasmid

#161621

Purpose

Overexpresses TMC5

Depositor

Mike McManus

Insert

TMC5 (TMC5 Human)

Tags

V5

Expression

Mammalian

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf6

Plasmid

#163598

Purpose

Expresses GDF-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf6 (Gdf6 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJL-mGold

Plasmid

#157997

Purpose

Expression of mGold in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold

Expression

Yeast

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

pTDH(GAP promoter)

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJH4531

Plasmid

#132366

Purpose

Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Tags

none

Expression

Worm

Promoter

Prgef-1

Available Since

Sept. 22, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSiren_EGFP-shLuc

Plasmid

#122287

Purpose

Knockdown control

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for Luc

Use

Retroviral

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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GalT-moxMaple3

Plasmid

#120884

Purpose

expression of moxMaple3 localized to the Golgi

Depositor

Erik Snapp

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Insert

moxMaple3

Tags

GalT signal anchor transmembrane domain

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pUM31dao

Plasmid

#87242

Purpose

Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants

Depositor

Anil Day

Article

Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.

Insert

D-amino oxidase

Expression

Plant

Promoter

plastid rrn

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6-mTFP1-His3MX6

Plasmid

#69850

Purpose

C-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET30b-7D-SrtA

Plasmid

#105600

Purpose

expresses 7D sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7D mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET30b-7Y-SrtA

Plasmid

#105601

Purpose

expresses 7Y sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7Y mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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SGL40C-H1.EFS.RFP657

Plasmid

#69148

Purpose

Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

sgRNA
EFS
tagRFP657

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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Construct 10 - U6p_40nt_stgRNA

Plasmid

#81249

Purpose

Expresses 40nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #1

Plasmid

#61515

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gcggcagctcctagctcagc

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pg50-2.11 (#65)

Plasmid

#35966

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Insert

Tie2 promoter-1st exon-1st intron

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only