We narrowed to 16,198 results for: grna

1. px330 EQR

   Plasmid

   #101733

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. BC1364-mouse U6-sgH2B

   Plasmid

   #164043

   Purpose

   U6-driven sgRNA targeting H2B

   Depositor

   Baohui Chen

   Article

   Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

   Insert

   H2B targeting sgRNA
   

   Use

   CRISPR

   Promoter

   mouse U6

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. MS2_adRNA

   Plasmid

   #124624

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with GFP

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MS2_adRNA_RAB7A
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330A_dCas9-1x6

   Plasmid

   #63600

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D1361Y

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330A_FokI-1x7

   Plasmid

   #63607

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-puro-cfRab8a_2

   Plasmid

   #26707

   Depositor

   Keith Mostov

   Article

   Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

   Insert

   dog Rab8a RNAi
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Mutation

   Dog Rab8a RNAi.

   Available Since

   Dec. 14, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pSUPER-retro-puro-shNup133-2

   Plasmid

   #87333

   Purpose

   To express shRNA against human Nup133

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   shRNA against human Nup133
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUPERIOR.puro-shREV3-4

   Plasmid

   #38029

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

   Insert

   shREV3-4 (REV3L human, Human, Mouse, Rat)
   

   Use

   RNAi

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pLL3.7 Lmo4.2 shRNA

   Plasmid

   #59293

   Purpose

   shRNA to mouse Lmo4

   Depositor

   Ulrike Heberlein

   Article

   Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24.

   Insert

   Lmo4.2 shRNA
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pLL3.7 Lmo4.3 shRNA

    Plasmid

    #59292

    Purpose

    shRNA to mouse Lmo4

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24.

    Insert

    Lmo4.3 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pEG302 22aa SunTag NtDRMcd SUP

    Plasmid

    #115489

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS316-RGR-GFP-mHDV

    Plasmid

    #51058

    Purpose

    Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS316-RGR-GFP-mHH

    Plasmid

    #51057

    Purpose

    Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. EE-SP!gIII

    Plasmid

    #48667

    Purpose

    Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Inserts

    Cas9
    anti-gIII tracrRNA
    

    Use

    CRISPR

    Promoter

    proC and tracrRNA promoter

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. SF-1 shRNA #1

    Plasmid

    #28186

    Depositor

    Bernard Schimmer

    Article

    Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

    Insert

    Nr5a1 shRNA (Nr5a1 Mouse)
    

    Use

    RNAi

    Tags

    cGFP

    Expression

    Mammalian

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pSuper 44-1

    Plasmid

    #51597

    Purpose

    shRNA 1 against rat and mouse Sema4D

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    shRNA1
    

    Use

    RNAi

    Promoter

    U6

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pLKO.1-blast-cfRab8b_3

    Plasmid

    #26709

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    dog Rab8b RNAi
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Mutation

    Dog Rab8b_3 RNAi.

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pRS423-SgH

    Plasmid

    #163972

    Purpose

    Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSP571

    Plasmid

    #139498

    Purpose

    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pGPD Cas9 / sgRNA (STL162)
    

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pGPD

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330A_FokI-1x6

    Plasmid

    #63606

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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